Hayles L B, McLintock J, Saunders J R
Can J Comp Med. 1972 Apr;36(2):83-8.
A Saskatchewan strain of the mosquito Culex tarsalis, transmitted a local strain of western equine encephalitis virus from chick to chick, between four and 44 days after an infective blood meal. At incubation temperatures of 69 and 75 degrees F, 120 transmissions occurred out of a possible 141, and all but seven of these were by single infected mosquitoes. At 75 degrees F virus titers in individual mosquitoes were more uniform and transmission was more efficient, than at 69 degrees F, although infection rates were similar at both temperatures. The minimum concentration of virus required to infect 50% of C.tarsalis was 10(2.5) intracerebral three-week old mouse LD(50) per 0.03 ml of donor blood. These findings provide direct evidence that C. tarsalis of Saskatchewan is a highly efficient vector of western equine encephalitis virus.
萨斯喀彻温省的一种致倦库蚊菌株，在吸食感染性血餐后4至44天内，将当地的西部马脑炎病毒株从一只小鸡传播到另一只小鸡。在69华氏度和75华氏度的孵化温度下，在可能的141次传播中有120次发生，其中除7次外均由单个感染的蚊子传播。在75华氏度时，单个蚊子体内的病毒滴度比在69华氏度时更均匀，传播效率更高，尽管在这两个温度下感染率相似。感染50%致倦库蚊所需的最低病毒浓度为每0.03毫升供血中含10(2.5)个脑内三周龄小鼠半数致死量。这些发现提供了直接证据，表明萨斯喀彻温省的致倦库蚊是西部马脑炎病毒的高效传播媒介。
