Carsten M E
Am J Obstet Gynecol. 1979 Mar 15;133(6):598-601. doi: 10.1016/0002-9378(79)90004-8.
A microsomal fraction was prepared from human pregnant uteri at term and at 6 to 19 weeks' gestation, and from nonpregnant uteri by differential centrifugation and purified on a discontinuous sucrose density gradient. This fraction bound calcium in the presence of adenosine triphosphate (ATP). ATP-dependent calcium binding in microsomal preparations was found to increase with advancing pregnancy. Addition of progesterone increased the ATP-dependent calcium binding, while addition of oxytocin decreased the ATP-dependent calcium binding. In combination, oxytocin and progesterone counteracted each other. The progesterone effect was specific for progesterone; three biologically inactive analogues had no effects on calcium binding. The actions of progesterone and of oxytocin on ATP-dependent calcium binding were found to be consistent with their respective in vivo uterine relaxing and contracting actions.
通过差速离心法从足月妊娠、妊娠6至19周的人妊娠子宫以及非妊娠子宫中制备微粒体部分，并在不连续蔗糖密度梯度上进行纯化。该部分在三磷酸腺苷（ATP）存在的情况下结合钙。发现微粒体制剂中依赖ATP的钙结合随着妊娠进展而增加。添加孕酮可增加依赖ATP的钙结合，而添加催产素则降低依赖ATP的钙结合。催产素和孕酮联合使用时相互抵消作用。孕酮的作用对孕酮具有特异性；三种无生物活性的类似物对钙结合无影响。发现孕酮和催产素对依赖ATP的钙结合的作用与其在体内子宫的舒张和收缩作用一致。
