Wu C H, Mennuti M T, Mikhail G
Am J Obstet Gynecol. 1979 Mar 15;133(6):666-72. doi: 10.1016/0002-9378(79)90016-4.
Ninety-seven amniotic fluid (AF) specimens, 67 with male fetuses and 30 with female fetuses, were obtained by amniocentesis at 14 to 18 weeks of gestation for analysis of free and protein-bound estrone (E1), estradiol (E2), androstenedione (A), testosterone (T), and progesterone (P). Ten maternal plasma (MP) samples of comparable gestational age were analyzed for the same free and protein-bound steroids. Significantly lower total E1, E2, A, and T, but not P, levels were found in AF when compared to MP. The percentage of free steroids was higher in AF than in MP, while the percentage of specific protein-bound steroids was lower in the AF than in MP. Free (unbound) steroid levels of E1 and P were higher in AF than in MP, whereas levels of E2 were lower in AF than in MP. The free A and T levels in MP were lower than in AF with male fetuses, yet higher than in AF with female fetuses. Possible biologic activity of steroids in this fluid may depend on the dynamic balance between the free, the specific protein-bound, and the nonspecific protein-bound steroids.
通过羊膜穿刺术获取了97份羊水（AF）样本，其中67份来自男性胎儿，30份来自女性胎儿，孕周为14至18周，用于分析游离型和蛋白结合型雌酮（E1）、雌二醇（E2）、雄烯二酮（A）、睾酮（T）和孕酮（P）。对10份孕周相当的孕妇血浆（MP）样本进行了相同的游离型和蛋白结合型类固醇分析。与MP相比，AF中总E1、E2、A和T水平显著降低，但P水平未降低。AF中游离类固醇的百分比高于MP，而AF中特异性蛋白结合类固醇的百分比低于MP。AF中E1和P的游离（未结合）类固醇水平高于MP，而AF中E2的水平低于MP。MP中游离A和T水平低于男性胎儿的AF，但高于女性胎儿的AF。该液体中类固醇的可能生物活性可能取决于游离型、特异性蛋白结合型和非特异性蛋白结合型类固醇之间的动态平衡。
