Bouldin T W, Cavanagh J B
Am J Pathol. 1979 Feb;94(2):253-70.
The early stages in the evolution of axonal degeneration in organophosphorous neuropathy have been studied by electron microscopy of single nerve fibers. Two young adult cats were given a single, intraperitoneal injection of di-isopropylfluorophosphate (DFP). Eighteen and 20 days later, respectively, after intracardiac perfusion with glutaraldehyde, single nerve fibers were teased from the cats' left recurrent laryngeal nerves. Four nerve fibers in progressive stages of axonal degeneration, were subserially sectioned and studied in the electron microscope. Unique, mid-internodal nerve fiber varicosities preceded an axonal degeneration which was initially focal and nonterminal. The varicosities were associated ultrastructurally with intra-axonal and/or intramyelinic vacuoles. Accumulations of axonal agranular reticulum were found in all four fibers but were associated with less than one half the varicosities. It appeared, both by light and electron microscopy, that the nerve fiber varicosities progressed to the focal, nonterminal axonal degeneration.
通过对单根神经纤维进行电子显微镜观察，研究了有机磷中毒性神经病中轴突变性演变的早期阶段。给两只成年猫腹腔内单次注射二异丙基氟磷酸酯（DFP）。分别在注射后18天和20天，用戊二醛进行心脏灌注后，从猫的左喉返神经中分离出单根神经纤维。对处于轴突变性进展阶段的4根神经纤维进行连续切片，并在电子显微镜下进行研究。独特的节间中部神经纤维膨出先于轴突变性，这种轴突变性最初是局灶性的且非终末性的。这些膨出在超微结构上与轴突内和/或髓鞘内空泡有关。在所有4根纤维中均发现了轴突无颗粒内质网的积聚，但与不到一半的膨出有关。通过光学显微镜和电子显微镜观察，似乎神经纤维膨出发展为局灶性、非终末性轴突变性。
