Prasad K N, Spuhler K, Arnold E B, Vernadakis A
In Vitro. 1979 Oct;15(10):807-12. doi: 10.1007/BF02618307.
The effect of various types of serum on morphological and biochemical changes in mouse neuroblastoma cells (clone NBP2) in culture was studied. The extent of spontaneous morphological differentiation varied markedly depending upon the type of serum and was maximal in agammaglobulin calf serum (CS). The extent of morphological differentiation after treatment of cells with cyclic AMP-stimulating agents was also dependent upon serum type and was least pronounced in fetal calf serum. The doubling time and extent of clumping varied with the type of serum. The activity of tyrosine hydroxylase (TH) in NB cells was dependent upon serum type and it was highest in newborn CS and agammaglobulin CS. Although elevation of intracellular levels of cyclic AMP in NBP2 clone invariably stimulates neurite formation and TH activity, these functions were increased in certain sera without a significant increase in the cellular cyclic AMP levels. The present study shows that neurite formation, growth rate and TH activity are regulated by more than one mode, one of which is mediated by cyclic AMP. The above changes are independently regulated in the sense that the expression of one can be increased in the absence of others.
研究了不同类型血清对培养的小鼠神经母细胞瘤细胞（克隆NBP2）形态和生化变化的影响。自发形态分化的程度因血清类型而异，在无丙种球蛋白小牛血清（CS）中最大。用环磷酸腺苷刺激剂处理细胞后，形态分化的程度也取决于血清类型，在胎牛血清中最不明显。倍增时间和聚集程度随血清类型而变化。神经母细胞瘤细胞中酪氨酸羟化酶（TH）的活性取决于血清类型，在新生CS和无丙种球蛋白CS中最高。虽然NBP2克隆中细胞内环磷酸腺苷水平的升高总是会刺激神经突形成和TH活性，但在某些血清中这些功能增强，而细胞内环磷酸腺苷水平没有显著增加。本研究表明，神经突形成、生长速率和TH活性受多种模式调节，其中一种由环磷酸腺苷介导。上述变化是独立调节的，即一种变化的表达可以在其他变化不存在的情况下增加。
