Rama B N, Prasad K N
Life Sci. 1984 May 21;34(21):2089-97. doi: 10.1016/0024-3205(84)90374-6.
The effect of heat in combination with DL-alpha-tocopheryl (vitamin E) succinate and adenosine 3', 5'-cyclic monophosphate (cAMP) stimulating agents on mouse neuroblastoma cells ( NBP2 ) in culture on the criterion of growth inhibition (due to cell death and inhibition of cell division) was studied. Heat (41 degrees-40 degrees) alone inhibited growth; however, the extent of growth inhibition was dependent upon the temperature and the time of heat treatment. Heat (41 degrees-40 degrees) in combination with vitamin E succinate (5 micrograms/ml) produced an additive effect on the criterion of growth inhibition. Vitamin C (100 micrograms/ml) failed to modify the effect of heat. Prostaglandin A2, a stimulator of adenylate cyclase, and 4 - (3-butoxy-4-methoxybenzyl)-2-imidazolidinone ( R020 -1724), an inhibitor of cyclic nucleotide phosphodiesterase, are known to induce irreversible differentiation in mouse neuroblastoma cells in culture. These agents, in combination with heat (40 degrees) produced a synergistic effect on the criterion of growth inhibition. These data suggest that the addition of vitamin E and cAMP stimulating agents may increase the effectiveness of hyperthermia protocol.
研究了热与DL-α-生育酚（维生素E）琥珀酸酯及腺苷3'，5'-环磷酸（cAMP）刺激剂联合使用对培养的小鼠神经母细胞瘤细胞（NBP2）生长抑制（因细胞死亡和细胞分裂抑制）标准的影响。单独热（41℃-40℃）可抑制生长；然而，生长抑制程度取决于温度和热处理时间。热（41℃-40℃）与维生素E琥珀酸酯（5微克/毫升）联合使用对生长抑制标准产生相加作用。维生素C（100微克/毫升）未能改变热的作用。已知腺苷酸环化酶刺激剂前列腺素A2和环核苷酸磷酸二酯酶抑制剂4-（3-丁氧基-4-甲氧基苄基）-2-咪唑烷酮（R020-1724）可诱导培养的小鼠神经母细胞瘤细胞发生不可逆分化。这些试剂与热（40℃）联合使用对生长抑制标准产生协同作用。这些数据表明，添加维生素E和cAMP刺激剂可能会提高热疗方案的有效性。
