Tjoeng F S, Tam J P, Merrifield R B
Int J Pept Protein Res. 1979;14(3):262-74. doi: 10.1111/j.1399-3011.1979.tb01932.x.
A fully protected 27--42 hexadecapeptide of the variable region of myeloma immunoglobulin M603 was synthesized on a 2-bromopropionyl-resin by the solid phase method. Side reactions due to cyclization of glycyl-2-oxypropionyl-resin were studied under different reaction conditions. The loss of peptide chains at the dipeptide and tripeptide stages due to diketopeperazine formation was also examined. These side reactions were circumvented by using a combination of fragment and stepwise coupling methods. The synthesized protected peptide was removed from the resin in 85% yield by photolysis, and purified by crystallization and by chromatography on a Sephadex LH-60 column.
采用固相法在2-溴丙酰树脂上合成了骨髓瘤免疫球蛋白M603可变区的一种完全保护的27 - 42十六肽。研究了在不同反应条件下甘氨酰-2-氧代丙酰树脂环化引起的副反应。还考察了在二肽和三肽阶段由于二酮哌嗪形成导致的肽链损失。通过片段法和逐步偶联法相结合避免了这些副反应。通过光解以85%的产率从树脂上除去合成的保护肽，并通过结晶和在Sephadex LH - 60柱上进行色谱纯化。
