Gulyas B J, Mattison D R
Anat Rec. 1979 Apr;193(4):863-82. doi: 10.1002/ar.1091930409.
Destruction of mouse oocytes in primordial and small primary follicles in response to treatment with 3-methylcholanthrene (MC) was studied at the ultrastructural level. Four-week old C57Bl/6N (B6) strain mice received a single injection of 80 mg/Kg MC in corn oil intraperitoneally. Controls received only corn oil. Ovaries from animals were prepared for light and electron microscopic examination at specified intervals after treatment. The number of primordial follicles remained constant in control animals. In contrast, their number decreased significantly (P less than 0.01) by three days, and they were depleted by seven days after MC treatment. Subtle degenerative modifications were noted in the ooplasm of primordial follicles two days after treatment. These changes consisted of vesiculation of mitochondrial cristae, increased electron density of the mitochondrial matrix, myelin structures in lipid droplets and in mitochondria. More advanced stages of degeneration of primordial follicles were characterized by further vesiculation or disappearance of mitochondrial cristae, chromatin clumping, and increased density of the ooplasm. Small primary follicles had undergone similar initial degeneration as primordial follicles. In more advanced stages of degeneration nuclear and cytoplasmic contents condensed, endoplasmic reticulum, Golgi complex and mitochondria swelled, small vesicles and multivesicular bodies appeared. In the most advanced stages of degeneration of small primary follicles it appeared that small portions of the oocyte were engulfed by the surrounding follicular cells. It is concluded that exposure of B6 mice to a single dose of MC results in atresia of oocytes in primordial and small primary follicles. Ultrastructurally, these degenerating oocytes of treated mice looked much like the spontaneously atretic oocytes in untreated animals.
在超微结构水平上研究了用3-甲基胆蒽（MC）处理后，原始卵泡和小初级卵泡中小鼠卵母细胞的破坏情况。4周龄的C57Bl/6N（B6）品系小鼠腹腔内单次注射80mg/Kg溶于玉米油的MC。对照组仅注射玉米油。在处理后的特定时间间隔，对动物的卵巢进行光镜和电镜检查。对照组动物的原始卵泡数量保持恒定。相比之下，MC处理后三天，其数量显著减少（P小于0.01），到七天时原始卵泡耗尽。处理后两天，在原始卵泡的卵质中观察到细微的退行性改变。这些变化包括线粒体嵴的空泡化、线粒体基质电子密度增加、脂滴和线粒体中的髓鞘样结构。原始卵泡更高级别的退化阶段表现为线粒体嵴进一步空泡化或消失、染色质凝聚以及卵质密度增加。小初级卵泡经历了与原始卵泡相似的初始退化过程。在更高级别的退化阶段，核内容物和细胞质内容物浓缩，内质网、高尔基体复合体和线粒体肿胀，出现小泡和多囊体。在小初级卵泡退化的最晚期阶段，似乎卵母细胞的小部分被周围的卵泡细胞吞噬。结论是，B6小鼠单次接触MC会导致原始卵泡和小初级卵泡中的卵母细胞闭锁。在超微结构上，处理过的小鼠中这些退化的卵母细胞与未处理动物中自然闭锁的卵母细胞非常相似。
