Charron A, Bebear C, Brun G, Yot P, Latrille J, Bove J M
J Bacteriol. 1979 Dec;140(3):763-8. doi: 10.1128/jb.140.3.763-768.1979.
The separation and partial characterization of two deoxyribonucleic acid polymerases from Spiroplasma citri have been achieved. The two enzymes had different elution properties on diethylaminoethyl (DEAE) cellulose and differed in their sensitivity to N-ethylmaleimide (NEM), preference for different template-primers, and sedimentation velocity in linear glycerol gradients. The first enzyme activity, ScA, was retained on DEAE-cellulose and was not inhibited by NEM. Activated deoxyribonucleic acid and poly(dA)-oligo(dT12) were the preferred template-primers. Arabinosyl-cytidine triphosphate had no effect. The sedimentation coefficient of ScA was 6.3s. The second activity, ScB, was not retained on DEAE-cellulose and was inhibited by NEM. Poly(dA)-oligo(dT12) was the preferred template-primer, whereas activated DNA was only poorly utilized. ScB was not affected by arabinosyl-cytidine triphosphate, and its sedimentation coefficient was 4.4s. The polymerization activities of the two enzymes were maximum at 37 to 40 degrees C.
已从柑橘螺原体中分离出两种脱氧核糖核酸聚合酶并对其进行了部分特性分析。这两种酶在二乙氨基乙基（DEAE）纤维素上具有不同的洗脱特性，对N-乙基马来酰亚胺（NEM）的敏感性、对不同模板引物的偏好以及在线性甘油梯度中的沉降速度也有所不同。第一种酶活性ScA保留在DEAE纤维素上，且不受NEM抑制。活化的脱氧核糖核酸和聚（dA）-寡聚（dT12）是其偏好的模板引物。阿糖胞苷三磷酸没有影响。ScA的沉降系数为6.3s。第二种活性ScB不保留在DEAE纤维素上，且受NEM抑制。聚（dA）-寡聚（dT12）是其偏好的模板引物，而活化的DNA利用率很低。ScB不受阿糖胞苷三磷酸的影响，其沉降系数为4.4s。这两种酶的聚合活性在37至40摄氏度时最高。
