Charron A, Castroviejo M, Bebear C, Latrille J, Bove J M
J Bacteriol. 1982 Mar;149(3):1138-41. doi: 10.1128/jb.149.3.1138-1141.1982.
Recently, two DNA polymerases (ScA and ScB) were isolated and characterized from Spiroplasma citri. We now have found a third DNA polymerase (ScC) not only in S. citri but also in the serologically related honeybee spiroplasma BC3 and the unrelated flower spiroplasma BNR1. Enzyme ScC is N-ethylmaleimide (NEM) sensitive. The three DNA polymerases from the honeybee spiroplasma seem to be similar to the respective enzymes of S. citri. However, whereas the NEM-resistant enzyme ScA from S. citri and that from the BC3 honeybee spiroplasma are retained on DEAE-cellulose and require 0.09 M KCl for elution, the NEM-resistant enzyme A from the flower spiroplasma BNR1 is not retained.
最近，从柑橘螺原体中分离并鉴定出了两种DNA聚合酶（ScA和ScB）。我们现在发现，不仅在柑橘螺原体中，而且在血清学相关的蜜蜂螺原体BC3和不相关的花卉螺原体BNR1中都存在第三种DNA聚合酶（ScC）。酶ScC对N-乙基马来酰亚胺（NEM）敏感。蜜蜂螺原体中的三种DNA聚合酶似乎与柑橘螺原体中的相应酶相似。然而，柑橘螺原体和蜜蜂螺原体BC3中的NEM抗性酶ScA保留在DEAE-纤维素上，洗脱需要0.09 M KCl，而花卉螺原体BNR1中的NEM抗性酶A则不被保留。
