δ-氨基乙酰丙酸脱水酶，粗糙脉孢菌血红素生物合成途径的调节酶。
Delta-aminolaevulinate dehydratase, the regulatory enzyme of the haem-biosynthetic pathway in Neurospora crassa.
作者信息
Muthukrishnan S, Malathi K, Padmanaban G
出版信息
Biochem J. 1972 Aug;129(1):31-7. doi: 10.1042/bj1290031.
Abstract
The activity of delta-aminolaevulinate dehydratase is very low in the mould Neurospora crassa compared with the activities detected in bacterial and animal systems. The enzyme is inducible in iron-deficient cultures by addition of iron and is repressed by protoporphyrin. The properties of the purified enzyme indicate its allosteric nature and susceptibility to feedback inhibition by coproporphyrinogen III. Neurospora extracts also contain a protein inhibitor of the enzyme and a small-molecule activator, which appears to be associated with the enzyme. The regulatory function of this enzyme in vivo is correlated with the accumulation of delta-aminolaevulinic acid in normal cultures of N. crassa. The decay curve of the iron-induced enzyme in vivo shows a biphasic pattern, with one of the components showing a half-life of 4-5 min.
摘要
与在细菌和动物系统中检测到的活性相比，在霉菌粗糙脉孢菌中，δ-氨基乙酰丙酸脱水酶的活性非常低。通过添加铁，该酶在缺铁培养物中可被诱导，并且被原卟啉抑制。纯化酶的特性表明其别构性质以及对粪卟啉原III反馈抑制的敏感性。粗糙脉孢菌提取物还含有该酶的一种蛋白质抑制剂和一种小分子激活剂，后者似乎与该酶相关。该酶在体内的调节功能与粗糙脉孢菌正常培养物中δ-氨基乙酰丙酸的积累相关。体内铁诱导酶的衰变曲线呈现双相模式，其中一个组分的半衰期为4 - 5分钟。
相似文献
1
Delta-aminolaevulinate dehydratase, the regulatory enzyme of the haem-biosynthetic pathway in Neurospora crassa.δ-氨基乙酰丙酸脱水酶，粗糙脉孢菌血红素生物合成途径的调节酶。
Biochem J. 1972 Aug;129(1):31-7. doi: 10.1042/bj1290031.
2
Purification, properties and synthesis of delta-aminolaevulinate dehydratase from Neurospora crassa.粗糙脉孢菌δ-氨基乙酰丙酸脱水酶的纯化、性质及合成
Biochem J. 1980 Oct 1;191(1):29-36. doi: 10.1042/bj1910029.
3
Characterization of protoporphyrin as the physiological regulatory of delta-aminolevulinate dehydratase in Neurospora crassa.原卟啉作为粗糙脉孢菌中δ-氨基乙酰丙酸脱水酶的生理调节剂的特性研究。
Biochim Biophys Acta. 1980 Apr 30;607(2):331-8. doi: 10.1016/0005-2787(80)90085-4.
4
Role of -aminolaevulinate dehydratase in the regulation of haem synthesis.δ-氨基乙酰丙酸脱水酶在血红素合成调节中的作用。
Biochem J. 1972 Jun;128(1):27P. doi: 10.1042/bj1280027pb.
5
Site of action of iron in the induction of delta-aminolevulinate dehydratase in Neurospora crassa.铁在粗糙脉孢菌中诱导δ-氨基乙酰丙酸脱水酶的作用位点。
Biochem Biophys Res Commun. 1977 Jul 11;77(1):35-41. doi: 10.1016/s0006-291x(77)80161-7.
6
Role of heme in the synthesis of cytochrome c oxidase in Neurospora crassa.血红素在粗糙脉孢菌细胞色素c氧化酶合成中的作用。
J Biol Chem. 1980 Dec 10;255(23):11130-4.
7
Regulation of heme biosynthesis in Neurspora crassa.粗糙脉孢菌中血红素生物合成的调控
J Biol Chem. 1969 Aug 10;244(15):4241-6.
8
Role of iron in the regulation of heme biosynthesis in Neurospora crassa.铁在粗糙脉孢菌血红素生物合成调控中的作用。
Biochem Biophys Res Commun. 1968 May 10;31(3):333-9. doi: 10.1016/0006-291x(68)90480-4.
9
10
Control of haem synthesis by feedback inhibition on human-erythrocyte delta-aminolaevulate dehydratase.通过对人红细胞δ-氨基-γ-酮戊酸脱水酶的反馈抑制来控制血红素合成。
Biochem J. 1966 Nov;101(2):550-2. doi: 10.1042/bj1010550.
引用本文的文献
1
Heme biosynthesis and its regulation: towards understanding and improvement of heme biosynthesis in filamentous fungi.血红素生物合成及其调控：深入了解和改善丝状真菌中的血红素生物合成。
Appl Microbiol Biotechnol. 2011 Aug;91(3):447-60. doi: 10.1007/s00253-011-3391-3. Epub 2011 Jun 18.
2
The Biosynthesis of delta-Aminolevulinic Acid in Higher Plants: I. Accumulation of delta-Aminolevulinic Acid in Greening Plant Tissues.高等植物中δ-氨基乙酰丙酸的生物合成：I. 绿色植物组织中δ-氨基乙酰丙酸的积累。
Plant Physiol. 1974 Feb;53(2):291-6. doi: 10.1104/pp.53.2.291.
3
Purification, properties and synthesis of delta-aminolaevulinate dehydratase from Neurospora crassa.粗糙脉孢菌δ-氨基乙酰丙酸脱水酶的纯化、性质及合成
Biochem J. 1980 Oct 1;191(1):29-36. doi: 10.1042/bj1910029.
4
Cold-induced increase of glycerol kinase activity in Neurospora crassa: rapid inactivation of the enzyme in vivo.粗糙脉孢菌中冷诱导甘油激酶活性增加：该酶在体内的快速失活
J Bacteriol. 1974 Nov;120(2):741-7. doi: 10.1128/jb.120.2.741-747.1974.
5
Molecular events during the release of delta-aminolevulinate dehydratase from catabolite repression.从分解代谢物阻遏中释放δ-氨基乙酰丙酸脱水酶期间的分子事件。
J Bacteriol. 1978 Jul;135(1):54-61. doi: 10.1128/jb.135.1.54-61.1978.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
delta-aminolaevulate dehydratase from Rhodopseudomonas spheroides.来自球形红假单胞菌的δ-氨基乙酰丙酸脱水酶
Biochem J. 1963 Jun;87(3):462-72. doi: 10.1042/bj0870462.
3
Initial stages in the biosynthesis of porphyrins. 2. The formation of delta-aminolaevulic acid from glycine and succinyl-coenzyme A by particles from chicken erythrocytes.卟啉生物合成的初始阶段。2. 鸡红细胞中的微粒体由甘氨酸和琥珀酰辅酶A形成δ-氨基乙酰丙酸。
Biochem J. 1958 Sep;70(1):71-81. doi: 10.1042/bj0700071.
4
Pbrphyrin biosynthesis in erythrocytes. II. Enzymes converting gamma-aminolevulinic acid to coproporphyrinogen.红细胞中的卟啉生物合成。II. 将γ-氨基乙酰丙酸转化为粪卟啉原的酶。
J Biol Chem. 1958 Jun;232(2):1119-40.
5
Concurrent decrease of enzymic activities concerned with the synthesis of coenzyme B 12 and of propionic acid in propionibacteria.丙酸杆菌中与辅酶B12合成及丙酸相关的酶活性同时降低。
Arch Biochem Biophys. 1967 Aug;121(2):304-10. doi: 10.1016/0003-9861(67)90080-x.
6
Purification and general properties of sigma-aminolaevulate dehydratase from cow liver.牛肝中σ-氨基乙酰丙酸脱水酶的纯化及一般性质
Biochem J. 1967 Jul;104(1):244-9. doi: 10.1042/bj1040244.
7
Biosynthesis of porphyrins in wheat leaves. II. 5-aminolaevulinate hydro-lyase.小麦叶片中卟啉的生物合成。II. 5-氨基乙酰丙酸水解酶
Can J Biochem. 1967 Feb;45(2):327-36. doi: 10.1139/o67-036.
8
Control of haem synthesis by feedback inhibition on human-erythrocyte delta-aminolaevulate dehydratase.通过对人红细胞δ-氨基-γ-酮戊酸脱水酶的反馈抑制来控制血红素合成。
Biochem J. 1966 Nov;101(2):550-2. doi: 10.1042/bj1010550.
9
Studies on iron metabolism in Neurospora crassa.粗糙脉孢菌中铁代谢的研究。
Arch Biochem Biophys. 1965 Jul;111(1):147-52. doi: 10.1016/0003-9861(65)90333-4.
10
Regulation of heme biosynthesis in Neurspora crassa.粗糙脉孢菌中血红素生物合成的调控
J Biol Chem. 1969 Aug 10;244(15):4241-6.
Zotero 插件