Cold-induced increase of glycerol kinase activity in Neurospora crassa: rapid inactivation of the enzyme in vivo.

The glycerol kinase activity induced by incubation of Neurospora crassa at low temperatures was rapidly lost when cultures were returned to 26 C. After a short lag, the activity disappeared irreversibly with a half-life of approximately 15 min. The loss of activity was not due to a change in the level of an inhibitor or activator. Glycerol reduced the activity loss but did not completely prevent it, which was an effect that was dependent on protein synthesis. The cold-induced activity was also always lost on addition of cycloheximide at all temperatures tested (0 to 26 C), which indicated continuous inactivation, although cycloheximide did not affect the actual rate of activity loss at 26 C. The basal glycerol kinase activity was not sensitive to cycloheximide. The mechanism responsible for inactivation was destroyed by sonic oscillation. The process is not thought to play a role in the cold-induced increase in activity. Glycerol kinase activity induced at 26 C by glycerol was also lost on addition of cycloheximide and after addition of sucrose.