Laanbroek H J, Smit A J, Nulend G K, Veldkamp H
Arch Microbiol. 1979 Jan 16;120(1):61-6. doi: 10.1007/BF00413275.
Clostridium cochlearium could be reproducibly enriched in an L-aspartate- and L-glutamate-limited, anaerobic chemostat inoculated with anaerobic sludge. L-glutamate, L-glutamine and L-histidine were the only fermentable substrates. Less specialised clostridia of the C. tetanomorphum type could only be isolated from batch enrichments with L-glutamate and L-aspartate as energy sources. Competition experiments with C. cochlearium and C. tetanomorphum in a L-glutamate-limited chemostat resulted in the selective elimination of the latter species. Addition of glucose to the medium resulted in coexistence of both species. The molar growth yields for L-glutamate at different dilution rates at 30 degrees C were determined for both species. The maximum specific growth rates on L-glutamate were 0.55 h-1 for C. cochlearium and 0.35 h-1 for C. tetanomorphum.
可通过接种厌氧污泥，在以L-天冬氨酸和L-谷氨酸为限制因素的厌氧恒化器中，可重复富集耳蜗梭菌。L-谷氨酸、L-谷氨酰胺和L-组氨酸是仅有的可发酵底物。破伤风样梭菌属中特异性较低的梭菌只能从以L-谷氨酸和L-天冬氨酸为能源的分批富集培养物中分离得到。在以L-谷氨酸为限制因素的恒化器中，对耳蜗梭菌和破伤风样梭菌进行竞争实验，结果导致后一种菌被选择性淘汰。向培养基中添加葡萄糖会使两种菌共存。测定了两种菌在30℃下不同稀释率时L-谷氨酸的摩尔生长产率。耳蜗梭菌对L-谷氨酸的最大比生长速率为0.55 h⁻¹，破伤风样梭菌为0.35 h⁻¹。
