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[借助氨银的DNA检测电子细胞化学方法]

[Electron-cytochemical method of DNA detection with the aid of ammonia silver].

作者信息

Erenpreis Ia G, Zirne R A

出版信息

Arkh Patol. 1979;41(1):57-60.

PMID:426641
Abstract

Three prescriptions for the detection of aldehyde groups of deoxyribose with ammoniac silver are proposed. The reaction can be carried out on tissue blocks or imprints; it is useful for DNA identification in the light and electron microscope, gives high contrast and allows to reveal DNA in structures with a relatively low content of DNA, for instance, in nucleoi. The disadvantages of the method include a possibility of nonspecific reduction of silver if the reaction is performed on a plastic-embedded material and damage of ultrastructures if the reaction is done prior to embedding.

摘要

提出了三种用氨银检测脱氧核糖醛基的方法。该反应可在组织块或印记上进行;对光镜和电镜下的DNA鉴定很有用,对比度高,能在DNA含量相对较低的结构(如核仁)中显示DNA。该方法的缺点包括:如果在塑料包埋材料上进行反应,可能会出现银的非特异性还原;如果在包埋前进行反应,则会破坏超微结构。

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