Fung K K, Kalant N
Biochem J. 1972 Sep;129(3):733-41. doi: 10.1042/bj1290733.
Phospholipids were found to be a constant component of rat glomerular basement-membrane preparations. The concentration fell during preparation of basement membrane by sonication of whole glomeruli, but then remained constant despite continued sonication. The proportions of the individual phospholipids were different from those of whole renal tissue or of isolated glomeruli. The basement-membrane preparations had no (Na(+)+K(+))-activated adenosine triphosphatase activity, an enzyme that is bound to plasma membranes. The concentration of lipid P was decreased on exposure in vivo or in vitro to antiserum against basement membrane; 7 days after injection of antiserum there was a change in the phospholipid composition, with a relative increase in phosphatidylcholine and a decrease in sphingomyelin content. The metabolic turnover rate of the lipid P remaining in the membrane was normal, as determined by (32)P incorporation. The loss of phospholipid was associated with decreases in the relative concentrations of hydroxyproline, hydroxylysine and glycine, and relative increases in proline, lysine, serine, threonine and valine. Administration of aminonucleoside and daunomycin produced proteinuria but did not cause a decrease in lipid P. Anticollagen and anti-lymphocyte sera that attached to the basement membrane but failed to produce proteinuria, also failed to affect the phospholipid content.
磷脂被发现是大鼠肾小球基底膜制剂的一种恒定成分。在通过对整个肾小球进行超声处理制备基底膜的过程中，其浓度下降，但随后尽管继续超声处理，浓度仍保持恒定。各个磷脂的比例与整个肾组织或分离的肾小球的比例不同。基底膜制剂没有（Na⁺ + K⁺）激活的腺苷三磷酸酶活性，这种酶与质膜结合。脂质P的浓度在体内或体外暴露于抗基底膜抗血清后降低；注射抗血清7天后，磷脂组成发生变化，磷脂酰胆碱相对增加，鞘磷脂含量减少。通过³²P掺入法测定，膜中剩余脂质P的代谢周转率正常。磷脂的损失与羟脯氨酸、羟赖氨酸和甘氨酸的相对浓度降低以及脯氨酸、赖氨酸、丝氨酸、苏氨酸和缬氨酸的相对增加有关。给予氨基核苷和柔红霉素会产生蛋白尿，但不会导致脂质P减少。附着于基底膜但未产生蛋白尿的抗胶原和抗淋巴细胞血清，也未影响磷脂含量。
