脑中转录核糖核酸的产后甲基化。前体转录核糖核酸甲基化的证据。
The postnatal methylation of transfer ribonucleic acid in brain. Evidence for the methylation of precursor transfer ribonucleic acid.
作者信息
Elahi E, Sellinger O Z
出版信息
Biochem J. 1979 Jan 1;177(1):381-4. doi: 10.1042/bj1770381.
Abstract
Incubation of 3-day-old rat brain with L-[methyl-3H]methionine resulted in the rapid labeling of low-molecular-weight cytoplasmic RNA. Electrophoresis in 15% polyacrylamide gels provided evidence for the methylation of precursor tRNA molecules, and high-performance liquid chromatography demonstrated N2-methylguanine to be the predominant methylated base formed during the first 2 min of labelling.
摘要
用L-[甲基-³H]甲硫氨酸培养3日龄大鼠脑，可使低分子量细胞质RNA快速标记。在15%聚丙烯酰胺凝胶中进行电泳，为前体tRNA分子的甲基化提供了证据，高效液相色谱表明N²-甲基鸟嘌呤是标记最初2分钟内形成的主要甲基化碱基。
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