Chiu D, Lubin B, Shohet S B
Br J Haematol. 1979 Feb;41(2):223-34. doi: 10.1111/j.1365-2141.1979.tb05851.x.
In order to study possible alterations in membrane lipids during sickling, we have measured the difference in susceptibility to lipid peroxidation, binding of trinitrobenzenesulfonic acid (TNBS) to aminophospholipids, and fatty acid uptake in cells containing sickle haemoglobin under aerobic and anaerobic conditions. We have also examined TNBS binding in irreversibly sickled cells in an attempt to evaluate the permanent effects of any such alterations. We found that when erythrocytes were sickled by deoxygenation, the susceptibility to lipid peroxidation and binding of TNBS to aminophospholipids was markedly increased, while normal control cells showed no change. These effects appeared to be specific for the sickled state rather than a nonspecific consequence of cell age or the concentration of sickle haemoglobin within the cell. In contrast, fatty acid incorporation into membrane phospholipids, representing potential lipid renewal, was decreased in the sickled state. Cell fractions enriched in irreversibly sickled cells showed increased TNBS labelling in air and only modest rises with anoxia. Taken together, these data imply a rearrangement of membrane lipids during the sickling process and suggest a permanent reorganization of membrane lipids in the irreversibly sickled cell.
为了研究镰状化过程中膜脂可能发生的变化，我们测定了在需氧和厌氧条件下，含镰状血红蛋白的细胞对脂质过氧化的敏感性差异、三硝基苯磺酸（TNBS）与氨基磷脂的结合情况以及脂肪酸摄取情况。我们还检测了不可逆镰状细胞中TNBS的结合情况，以评估此类变化的永久性影响。我们发现，当红细胞因脱氧而发生镰状化时，对脂质过氧化的敏感性以及TNBS与氨基磷脂的结合显著增加，而正常对照细胞则无变化。这些影响似乎是镰状化状态所特有的，而非细胞年龄或细胞内镰状血红蛋白浓度的非特异性结果。相比之下，代表潜在脂质更新的脂肪酸掺入膜磷脂的过程在镰状化状态下减少。富含不可逆镰状细胞的细胞组分在空气中显示出TNBS标记增加，而在缺氧时仅略有升高。综合这些数据表明，在镰状化过程中膜脂发生了重排，并提示在不可逆镰状细胞中膜脂存在永久性重组。
