新生犊牛腹泻病毒的理化特性
Physiochemical characterization of a neonatal calf diarrhea virus.
作者信息
Welch A B, Thompson T L
出版信息
Can J Comp Med. 1973 Jul;37(3):295-301.
Physicochemical characteristics of two isolates of a neonatal calf diarrhea virus were investigated. Neither isolate was sensitive to ether or chloroform, both were stable at pH 3.0, were relatively heat resistant, but were thermolabile when heated to 50 degrees C for one hour in the presence of 1.0 M MgCl(2). Multiplication of virus was not inhibited by concentrations of 5-iodo-2'-deoxyuridine (IDUR) up to 500 microg/ml, which indicated that the nucleic acid was ribonucleic acid (RNA). Also, multiplication was not inhibited by concentrations of actinomycin D (AD) up to 0.5 microg/ml. Thermal denaturation studies demonstrated that the nucleic acid had a high melting temperature. Resistance to lipid solvents, stability at an acid pH, relatively high thermostability, type of nucleic acid, plus previous reports from this laboratory on general morphology and cytopathogenicity suggest that the virus may belong to the diplornavirus (reovirus) group. However, thermolability in the presence of 1.0 M MgCl(2) is not consistent with characteristics of this group.
对两株新生小牛腹泻病毒的理化特性进行了研究。两株病毒均对乙醚或氯仿不敏感，在pH 3.0时均稳定，相对耐热，但在1.0 M氯化镁存在下加热至50℃ 1小时则热不稳定。浓度高达500μg/ml的5-碘-2'-脱氧尿苷（IDUR）不抑制病毒增殖，这表明核酸为核糖核酸（RNA）。此外，浓度高达0.5μg/ml的放线菌素D（AD）也不抑制病毒增殖。热变性研究表明该核酸具有较高的解链温度。对脂质溶剂的抗性、在酸性pH下的稳定性、相对较高的热稳定性、核酸类型，再加上本实验室先前关于一般形态和细胞致病性的报道，提示该病毒可能属于双RNA病毒（呼肠孤病毒）科。然而，在1.0 M氯化镁存在下的热不稳定性与该科的特性不一致。
Zotero 插件