维生素B12缺乏的乳鼠肝脏和大脑中的脂质组成与代谢

Lipid composition and metabolism in liver and brain of vitamin B12-deficient rat sucklings.

Akesson B, Fehling C, Jägerstad M

Br J Nutr. 1979 Mar;41(2):263-74. doi: 10.1079/bjn19790035.

Abstract

Rat sucklings (18-d-old) bred from vitamin B12-deprived dams were compared with vitamin B12-supplemented dams' offspring, which were considered normal rat sucklings. 2. The vitamin B12-deficient rat sucklings had lower body-weight, liver weight and brain weight. 3. Vitamin B12 deficiency was also evident from the tenfold lower concentrations of vitamin B12 in liver and cerebellum. 4. The concentration of liver lipid was markedly increased in vitamin B12-deficient rats; triacylglycerol accounted for most of the increase. In brain the lipid concentration was slightly decreased (less than 0.05). 5. The methylation of ethanolamine phosphoglyceride to choline phosphoglyceride was reduced in both liver and brain in vitamin B12-deficient rats, as measured after the administration of [14C]ethanolamine. A slight decrease in choline phosphoglyceride concentration could be a consequence of this finding. The composition of phospholipids was otherwise normal. 6. Odd-chain fatty acids (pentadecanoate and heptadecanoate) accumulated in both liver and brain of the vitamin B12-deficient rat sucklings and constituted approximately 1% of total fatty acid. 7. The biosynthesis of fatty acid and cholesterol from intraperitoneally-injected 3H2O and [14C]propionate was unchanged in vitamin B12 deficiency.

摘要

将维生素B12缺乏的母鼠所哺育的18日龄乳鼠与补充了维生素B12的母鼠的后代（视为正常乳鼠）进行比较。2. 维生素B12缺乏的乳鼠体重、肝脏重量和脑重量较低。3. 肝脏和小脑维生素B12浓度降低十倍也表明存在维生素B12缺乏。4. 维生素B12缺乏的大鼠肝脏脂质浓度显著增加；三酰甘油占增加量的大部分。脑中脂质浓度略有下降（小于0.05）。5. 给予[14C]乙醇胺后测定发现，维生素B12缺乏的大鼠肝脏和脑中乙醇胺磷酸甘油酯甲基化为胆碱磷酸甘油酯的过程均减少。胆碱磷酸甘油酯浓度略有下降可能是这一发现的结果。磷脂的组成在其他方面正常。6. 维生素B12缺乏的乳鼠肝脏和脑中奇数链脂肪酸（十五烷酸和十七烷酸）积累，约占总脂肪酸的1%。7. 维生素B12缺乏时，腹腔注射3H2O和[14C]丙酸盐后脂肪酸和胆固醇的生物合成未发生变化。