Fehling C, Jägerstad M, Arvidson G
Nutr Metab. 1978;22(2):82-9. doi: 10.1159/000176201.
Rats were deprived of vitamin B12 in order to study the effect of this deprivation on the metabolism of lipids in the liver and the nervous system. Serum vitamin B12 concentrations of 102.7 and 78 pg/ml were found at sacrifice after 5 and 6 months, respectively. Neurological testing failed to reveal signs of neuropathy. The total liver lipids were decreased in the vitamin-B12-deprived animals, but no changes were detected in the lipid concentration or in the phospholipid composition of the nervous system. Some animals were given propionate, and in these, contrary to expectations, pentadecanoic acid and heptadecanoic acid were found in smaller amounts in the liver triglycerides of the vitamin B12-deprived rats than in the control rats. This could be due to the inhibitory effect of methylmalonyl CoA on fatty-acid synthesis, demonstrated by others in vitro.
为了研究维生素B12缺乏对肝脏和神经系统脂质代谢的影响，对大鼠进行了维生素B12缺乏处理。在处死时，分别在5个月和6个月后发现血清维生素B12浓度为102.7和78 pg/ml。神经学测试未发现神经病变迹象。维生素B12缺乏的动物肝脏总脂质减少，但神经系统的脂质浓度或磷脂组成未检测到变化。给一些动物喂食丙酸盐，与预期相反，在维生素B12缺乏大鼠的肝脏甘油三酯中，十五烷酸和十七烷酸的含量比对照大鼠少。这可能是由于甲基丙二酰辅酶A对脂肪酸合成的抑制作用，其他人已在体外证明了这一点。
