Schueler R L, Morehouse L G, Olson L D
Can J Comp Med. 1973 Oct;37(4):327-9.
A rapid and specific direct fluorescent antibody test was developed for the identification of group E Streptococci (GES). Tests for specificity included an inhibition test and application of the conjugate to smears of Staphylococcus aureus, Pasteurella multocida, and streptococci of Lancefield's groups, A, B, C, D, E (types I, IV and untypeable), F and G. With the inhibition test, a specific blocking reaction occurred and the conjugate cross-reacted only with group C Streptococci (GCS). Adsorption with GCS eliminated the cross-reaction and rendered the conjugate specific for GES.
开发了一种快速、特异的直接荧光抗体检测方法用于鉴定E群链球菌(GES)。特异性检测包括抑制试验,以及将结合物应用于金黄色葡萄球菌、多杀巴斯德菌和兰斯菲尔德A、B、C、D、E(I型、IV型和无法分型)、F和G群链球菌的涂片。通过抑制试验,发生了特异性阻断反应,且结合物仅与C群链球菌(GCS)发生交叉反应。用GCS吸附可消除交叉反应,使结合物对GES具有特异性。