Pavlova M T, Beauvais E, Brezenski F T, Litsky W
Appl Microbiol. 1972 Mar;23(3):571-7. doi: 10.1128/am.23.3.571-577.1972.
Fluorescent-antibody (FA) techniques were employed in an attempt to develop a rapid test for the identification of group D streptococci. Fresh isolates were obtained from sewege and feces of sheep, cattle, horses, rabbits, chickens, geese, and rats. Identification to species were made by the conventional physiological, biochemical, and serological tests. Both whole and disrupted cells of representative strains of each species were used for the preparation of the group D streptococcus vaccine. Globulin fractions of individual and pooled antisera were labeled with fluorescein isothiocyanate, and the resulting conjugates were tested with homologous and heterologous antigens. The specificity of the conjugates and staining was assessed by adsorption and inhibition tests utilizing controls with homologous and heterologous antigens. Employing the direct staining method and individual and pooled conjugates, it was possible to obtain 84 and 85% positive FA reactions, respectively, with group D streptococcal strains. Trypsinization of the smears prior to staining eliminated all FA cross-reactions observed with non-group D streptococci and staphylococci. These findings suggest that the direct staining method will be of value in the rapid identification of group D streptococci.
荧光抗体（FA）技术被用于尝试开发一种用于鉴定D群链球菌的快速检测方法。从绵羊、牛、马、兔、鸡、鹅和大鼠的污水和粪便中获取新鲜分离株。通过传统的生理、生化和血清学检测进行种属鉴定。每个种属的代表性菌株的完整细胞和破碎细胞都用于制备D群链球菌疫苗。用异硫氰酸荧光素标记个体和混合抗血清的球蛋白组分，并用同源和异源抗原检测所得的结合物。通过使用同源和异源抗原对照的吸附和抑制试验评估结合物和染色的特异性。采用直接染色法以及个体和混合结合物，分别对D群链球菌菌株获得了84%和85%的阳性FA反应。染色前对涂片进行胰蛋白酶处理消除了所有观察到的与非D群链球菌和葡萄球菌的FA交叉反应。这些发现表明直接染色法在快速鉴定D群链球菌方面将具有价值。
