Martinson H G, True R, Lau C K, Mehrabian M
Biochemistry. 1979 Mar 20;18(6):1075-82. doi: 10.1021/bi00573a022.
The contact-site cross-linkers tetranitromethane, UV light, formaldehyde, and a monofunctional imido ester have been used to generate a collection of histone-histone dimers and trimers from nuclei and chromatin. Four different H2B-H4 dimers have been isolated. Preliminary CNBr peptide mapping has shown that all are cross-linked at different positions that are apparently clustered within the C-terminal regions of these histones. Similarily, two different H2A-H2B dimers and two different H2A-H2B-H4 trimers have been partially characterized. The data suggest a functional map for H2B in which the N-terminal third interacts with DNA, the middle third interacts with H2A, and the C-terminal third interacts with H4. We hope, by pursuing this type of analysis, to develop a detailed understanding of each histone-histone binding interaction through saturation cross-linking of the binding sites.
接触位点交联剂四硝基甲烷、紫外线、甲醛和一种单功能亚氨基酯已被用于从细胞核和染色质中生成一组组蛋白-组蛋白二聚体和三聚体。已分离出四种不同的H2B-H4二聚体。初步的溴化氰肽图谱显示，所有这些二聚体都在不同位置交联，这些位置显然聚集在这些组蛋白的C端区域内。同样，两种不同的H2A-H2B二聚体和两种不同的H2A-H2B-H4三聚体也已得到部分表征。数据表明了H2B的功能图谱，其中N端三分之一与DNA相互作用，中间三分之一与H2A相互作用，C端三分之一与H4相互作用。我们希望通过进行这种类型的分析，通过结合位点的饱和交联来深入了解每种组蛋白-组蛋白结合相互作用。
