γ 射线辐照流感病毒可独特地诱导 IFN-I 介导的淋巴细胞活化，而不依赖 TLR7/MyD88 途径。

Gamma-irradiated influenza virus uniquely induces IFN-I mediated lymphocyte activation independent of the TLR7/MyD88 pathway.

机构信息

Department of Emerging Pathogens and Vaccines, The John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory, Australia.

出版信息

PLoS One. 2011;6(10):e25765. doi: 10.1371/journal.pone.0025765. Epub 2011 Oct 5.

BACKGROUND

We have shown previously in mice, that infection with live viruses, including influenza/A and Semliki Forest virus (SFV), induces systemic partial activation of lymphocytes, characterized by cell surface expression of CD69 and CD86, but not CD25. This partial lymphocytes activation is mediated by type-I interferons (IFN-I). Importantly, we have shown that γ-irradiated SFV does not induce IFN-I and the associated lymphocyte activation.

PRINCIPAL FINDINGS

Here we report that, in contrast to SFV, γ-irradiated influenza A virus elicits partial lymphocyte activation in vivo. Furthermore, we show that when using influenza viruses inactivated by a variety of methods (UV, ionising radiation and formalin treatment), as well as commercially available influenza vaccines, only γ-irradiated influenza virus is able to trigger IFN-I-dependent partial lymphocyte activation in the absence of the TLR7/MyD88 signalling pathways.

CONCLUSIONS

Our data suggest an important mechanism for the recognition of γ-irradiated influenza vaccine by cytosolic receptors, which correspond with the ability of γ-irradiated influenza virus to induce cross-reactive and cross-protective cytotoxic T cell responses.

背景

我们之前在小鼠中表明，包括流感病毒/A 和 Semliki Forest 病毒 (SFV) 在内的活病毒感染会诱导全身淋巴细胞部分激活，其特征为细胞表面表达 CD69 和 CD86，但不表达 CD25。这种部分淋巴细胞激活是由 I 型干扰素 (IFN-I) 介导的。重要的是，我们已经表明，γ 射线辐照的 SFV 不会诱导 IFN-I 和相关的淋巴细胞激活。

主要发现

在这里，我们报告说，与 SFV 相反，γ 射线辐照的流感病毒在体内引发部分淋巴细胞激活。此外，我们表明，当使用通过多种方法（UV、电离辐射和福尔马林处理）灭活的流感病毒以及市售流感疫苗时，只有 γ 射线辐照的流感病毒能够在没有 TLR7/MyD88 信号通路的情况下触发 IFN-I 依赖性部分淋巴细胞激活。