Dennis J, Kisilevsky R
Biochim Biophys Acta. 1979 Feb 27;561(2):421-34. doi: 10.1016/0005-2787(79)90150-3.
Three fractions of poly(A)-containing RNA were separated from total rat liver RNA using poly(U)-Sepharose 4B affinity chromatography. The poly(A)-containing RNA fractions were released by thermal elution. Fraction 1, eluted under the mildest conditions, and had poly(A) tracts of approx. 200 AMP units in length which appeared to be associated with poly(U) sequences of 20-50 UMP in length. Fraction 1 appeared to be present mainly in the nucleus and, its size distribution was similar to that of fractions 2 and 3. Fractions 2 and 3 eluted at higher temperatures and were associated mainly with polysomal and microsomal fractions. Poly(U) sequences were absent in fractions 2 and 3 while their poly(A) sequences had a size distribution characteristic of those reported in the mRNA of other organisms.
使用聚（U）-琼脂糖4B亲和色谱法从大鼠肝脏总RNA中分离出三个含多聚腺苷酸（poly(A)）的RNA组分。通过热洗脱释放含poly(A)的RNA组分。组分1在最温和的条件下洗脱，其poly(A)序列长度约为200个腺苷酸单位，似乎与长度为20 - 50个尿苷酸的聚（U）序列相关。组分1似乎主要存在于细胞核中，其大小分布与组分2和3相似。组分2和3在较高温度下洗脱，主要与多核糖体和微粒体组分相关。组分2和3中不存在聚（U）序列，而它们的poly(A)序列具有其他生物体mRNA中报道的大小分布特征。
