Trachsel H, Erni B, Schreier M H, Braun L, Staehelin T
Biochim Biophys Acta. 1979 Feb 27;561(2):484-90. doi: 10.1016/0005-2787(79)90156-4.
Seven protein synthesis initiation factors were isolated from Krebs II ascites cells using the procedures developed for the purification of the corresponding factors from rabbit reticulocytes. The ascites factors display identical characteristics in ion exchange chromatography and sucrose density gradient sedimentation. Based on their profiles in SDS polyacrylamide gels, the ascites factors have polypeptide profiles and molecular weights identical to those of the reticulocyte factors. Most significantly, each ascites factor is competent in replacing its corresponding reticulocyte factor in a reconstituted in vitro protein synthesizing system which is dependent on all seven factors.
利用从兔网织红细胞中纯化相应因子所开发的方法，从克雷布斯II腹水细胞中分离出七种蛋白质合成起始因子。这些腹水因子在离子交换色谱和蔗糖密度梯度沉降中表现出相同的特性。根据它们在SDS聚丙烯酰胺凝胶中的图谱，腹水因子的多肽图谱和分子量与网织红细胞因子相同。最显著的是，在依赖于所有七种因子的重组体外蛋白质合成系统中，每种腹水因子都能够替代其相应的网织红细胞因子。
