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相似文献

1
Myosin ATP hydrolysis: a mechanism involving a magnesium chelate complex.肌球蛋白ATP水解：一种涉及镁螯合物复合体的机制。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3793-6. doi: 10.1073/pnas.70.12.3793.
2
Cooperative role of two sulfhydryl groups in myosin adenosine triphosphatase.两个巯基在肌球蛋白三磷酸腺苷酶中的协同作用。
Biochemistry. 1974 May 7;13(10):2014-22. doi: 10.1021/bi00707a003.
3
Enzymatic studies on the interaction of myosin and heavy meromyosin with 1,N 6 -ethenoadenosine triphosphate ( ATP), a fluorescent analog of ATP.关于肌球蛋白和重酶解肌球蛋白与1,N⁶-乙烯基三磷酸腺苷（ATP）（ATP的一种荧光类似物）相互作用的酶学研究。
Biochem Biophys Res Commun. 1973 Feb 5;50(3):926-33. doi: 10.1016/0006-291x(73)91334-x.
4
[Study of the magnesium inhibition of the myosin ATPase reaction].[镁对肌球蛋白ATP酶反应抑制作用的研究]
Biokhimiia. 1974 Nov-Dec;39(6):1285-92.
5
The sulfhydryl groups involved in the active site of myosin B adenosinetriphosphatase. II. Effect of modification of the Sa thiol group on superprecipitation and clearing.
J Biochem. 1977 Apr;81(4):933-9. doi: 10.1093/oxfordjournals.jbchem.a131558.
6
Theoretical studies of the ATP hydrolysis mechanism of myosin.肌球蛋白ATP水解机制的理论研究。
Biophys J. 2001 Nov;81(5):2786-94. doi: 10.1016/S0006-3495(01)75921-8.
7
Interaction of adipic acid dihydrazide analogue of ATP with myosin. Involvement of the essential sulfhydryl groups.
Biochemistry. 1977 May 31;16(11):2532-8. doi: 10.1021/bi00630a033.
8
The characterization of myosin-product complexes and of product-release steps during the magnesium ion-dependent adenosine triphosphatase reaction.在镁离子依赖性三磷酸腺苷酶反应过程中肌球蛋白 - 产物复合物及产物释放步骤的表征。
Biochem J. 1974 Aug;141(2):331-49. doi: 10.1042/bj1410331.
9
Electron spin resonance of myosin spin labeled at the S1 thiol groups during hydrolysis of adenosine triphosphate.三磷酸腺苷水解过程中在S1巯基处标记自旋的肌球蛋白的电子自旋共振。
Arch Biochem Biophys. 1973 Oct;158(2):853-63. doi: 10.1016/0003-9861(73)90581-x.
10
An activation mechanism for ATP cleavage in muscle.肌肉中ATP水解的激活机制。
J Supramol Struct. 1975;3(2):112-24. doi: 10.1002/jss.400030204.

引用本文的文献

1
Switch-1 instability at the active site decouples ATP hydrolysis from force generation in myosin II.活性位点的 Switch-1 不稳定性使肌球蛋白 II 的 ATP 水解与力产生解耦。
Cytoskeleton (Hoboken). 2021 Jan;78(1):3-13. doi: 10.1002/cm.21650. Epub 2021 Jan 11.
2
Age affects myosin relaxation states in skeletal muscle fibers of female but not male mice.年龄会影响雌性而非雄性小鼠骨骼肌纤维中的肌球蛋白松弛状态。
PLoS One. 2018 Sep 18;13(9):e0199062. doi: 10.1371/journal.pone.0199062. eCollection 2018.
3
Identification and characterization of Myosin from rat testicular peritubular myoid cells.大鼠睾丸生精小管肌样细胞中肌球蛋白的鉴定与特性分析
Biol Reprod. 2008 Dec;79(6):1210-8. doi: 10.1095/biolreprod.107.066472. Epub 2008 Aug 20.
4
Evidence for an altered structure of actin-S1 complexes when Mg-adenylylimidodiphosphate binds.当镁-腺苷酰亚胺二磷酸结合时，肌动蛋白-S1复合物结构改变的证据。
J Muscle Res Cell Motil. 1980 Sep;1(3):305-20. doi: 10.1007/BF00711933.
5
Unusual features of the Ca2+-ATPase activity of myosin from fast skeletal muscle of the frog: effect of actin and SH1 thiol group modification.
J Muscle Res Cell Motil. 1983 Apr;4(2):191-206. doi: 10.1007/BF00712030.
6
Formation of ATP-insensitive weakly-binding crossbridges in single rabbit psoas fibers by treatment with phenylmaleimide or para-phenylenedimaleimide.通过用苯基马来酰亚胺或对苯二马来酰亚胺处理，在单个兔腰大肌纤维中形成对ATP不敏感的弱结合横桥。
Biophys J. 1992 Feb;61(2):358-67. doi: 10.1016/S0006-3495(92)81842-8.
7
Active site trapping of nucleotides by crosslinking two sulfhydryls in myosin subfragment 1.通过交联肌球蛋白亚片段1中的两个巯基对核苷酸进行活性位点捕获。
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4966-70. doi: 10.1073/pnas.76.10.4966.

本文引用的文献

1
ON THE ACTIVATION OF MYOSIN ATPASE BY EDTA.
Biochim Biophys Acta. 1964 Jul 8;89:186-8.
2
THE ANTAGONISTIC ACTION OF MAGNESIUM IONS AND ETHYLENEDIAMINETETRAACETATE ON MYOSIN A ATPASE (POTASSIUM ACTIVATED).
Biochim Biophys Acta. 1964 Sep 18;89:566-9. doi: 10.1016/0926-6569(64)90090-2.
3
On the role of calcium in the activity of adenosine 5'-triphosphate hydrolysis by actomyosin.论钙在肌动球蛋白水解腺苷5'-三磷酸活性中的作用。
J Biol Chem. 1959 Oct;234:2764-9.
4
The hydrolysis of purine and pyrimidine nucleoside triphosphates by myosin.
J Biol Chem. 1956 Mar;219(1):95-101.
5
The relationship between sulfhydryl groups and the activation of myosin adenosinetriphosphatase.巯基与肌球蛋白三磷酸腺苷酶激活之间的关系。
J Biol Chem. 1956 Feb;218(2):653-9.
6
Thiolysis of some dinitrophenyl derivatives of amino acids.氨基酸某些二硝基苯基衍生物的硫解作用
Biochem Biophys Res Commun. 1967 Oct 26;29(2):178-83. doi: 10.1016/0006-291x(67)90583-9.
7
Actin-heavy meromyosin biding. Determination of binding stoichiometry from adenosine triphosphatase kinetic measurements.肌动蛋白-重酶解肌球蛋白结合。通过三磷酸腺苷酶动力学测量确定结合化学计量。
Biochemistry. 1970 Jun 9;9(12):2402-8. doi: 10.1021/bi00814a003.
8
A simple procedure for the preparation of tropomyosin-free F-actin.
J Mechanochem Cell Motil. 1972 Dec;1(4):189-90.
9
The effects of calcium and magnesium ions on the adenosine triphosphatase and inosine triphosphatase activities of myosin A.钙和镁离子对肌球蛋白A的三磷酸腺苷酶和三磷酸肌苷酶活性的影响。
Biochem J. 1969 Aug;113(5):821-7. doi: 10.1042/bj1130821.
10
The binding of ADP to myosin.二磷酸腺苷与肌球蛋白的结合。
Biochim Biophys Acta. 1969 Jan 14;172(1):158-70. doi: 10.1016/0005-2728(69)90101-7.

肌球蛋白ATP水解：一种涉及镁螯合物复合体的机制。

Myosin ATP hydrolysis: a mechanism involving a magnesium chelate complex.

作者信息

Burke M, Reisler E, Harrington W F

出版信息

Proc Natl Acad Sci U S A. 1973 Dec;70(12):3793-6. doi: 10.1073/pnas.70.12.3793.

DOI:10.1073/pnas.70.12.3793
PMID:4272702
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC427330/
Abstract

It is suggested that under physiological conditions (> 1 mM Mg(2+)) MgATP binds to myosin to form a chelate involving the two reactive sulfhydryl sites (SH(1) and SH(2)). The stability of the chelate structure results in marked inhibition of the myosin ATPase in the presence of millimolar magnesium ion. The inhibitory effect of magnesium ion can be eliminated chemically by blocking either the SH(1) or SH(2) site since this precludes formation of the chelate. In muscle, actin apparently behaves in a similar fashion in that its interaction with myosin causes a disruption of the chelate structure.

摘要

有人提出，在生理条件下（>1 mM Mg(2+)），MgATP与肌球蛋白结合形成一种螯合物，涉及两个反应性巯基位点（SH(1)和SH(2)）。螯合物结构的稳定性导致在存在毫摩尔镁离子的情况下肌球蛋白ATP酶受到显著抑制。通过阻断SH(1)或SH(2)位点，可以化学消除镁离子的抑制作用，因为这会阻止螯合物的形成。在肌肉中，肌动蛋白的行为显然类似，即它与肌球蛋白的相互作用会导致螯合物结构的破坏。