哮喘儿童白细胞中三磷酸腺苷酶增加。
Increased adenosine triphosphatase in leukocytes of asthmatic children.
作者信息
Coffey R G, Hadden J W, Middleton E
出版信息
J Clin Invest. 1974 Jul;54(1):138-46. doi: 10.1172/JCI107735.
Adenosine triphosphatase (ATPase) activities were compared in leukocytes of asthmatic and nonasthmatic children. Both Mg(2+)- and Ca(2+)-dependent ATPase activities were significantly elevated in two membrane fractions (59 to 66%) and in a superntant fraction (68 to 72%) prepared from sonicated leukocytes of asthmatic subjects. Intact cell surface or ecto ATPase was also elevated (67 to 76%) in asthmatic leukocytes. Alternate day glucocorticosteroid therapy was associated with leukocyte ATPase activities intermediate between those for asthmatics not receiving steroids and for nonasthmatic control subjects. Incubation of normal leukocytes with 10(-8) M hydrocortisone or leukocyte membranes with 10(-4)-10(-3) M hydrocortisone in vitro also resulted in decreased ATPase activities. The elevated leukocyte ATPase activities appear to relate to the adrenergic imbalance in asthma previously characterized by reduced beta adrenergic responsiveness of adenylate cyclase and suggest the possibility of more than one enzymatic abnormality intrinsic to the asthmatic condition.
对哮喘儿童和非哮喘儿童白细胞中的三磷酸腺苷酶（ATP酶）活性进行了比较。在从哮喘患者经超声处理的白细胞制备的两个膜组分（59%至66%）和一个上清液组分（68%至72%）中，镁离子（Mg²⁺）依赖性和钙离子（Ca²⁺）依赖性ATP酶活性均显著升高。哮喘患者白细胞中完整的细胞表面或外切ATP酶也升高（67%至76%）。隔日糖皮质激素治疗与白细胞ATP酶活性相关，其活性介于未接受类固醇治疗的哮喘患者和非哮喘对照受试者之间。在体外，用10⁻⁸ M氢化可的松孵育正常白细胞或用10⁻⁴ - 10⁻³ M氢化可的松孵育白细胞膜，也会导致ATP酶活性降低。白细胞ATP酶活性升高似乎与哮喘中先前以腺苷酸环化酶的β肾上腺素能反应性降低为特征的肾上腺素能失衡有关，并提示哮喘状态存在不止一种内在酶异常的可能性。
Zotero 插件