Carter E I, Valli V E, McSherry B J, Milne F J, Robinson G A, Lumsden J H
Can J Comp Med. 1974 Jul;38(3):303-13.
Three Standardbred horses were given 0.2 mg (1 mCi) of (75)selenomethionine intravenously and a second group of three were given 10 mCi of tritiated diisopropylfluorophosphate (0.5 mg) intravenously. Observations on labeled cells were continued for 250 days after radioselenium injection and 160 days after tritium injection. The lifespan of erythrocytes using (75)selenmethionine was 155 +/- 10 days and 148 +/- 7.8 days using tritiated diisopropylfluorophosphate. There was no significant difference at the 10% level between the lifespans, using these labels. The uptake of radioselenium into erythrocytes reached a mean maximum of 11.5% at 82 +/- 18.9 days after injection of the label. There was an elution of from 17.6% of the injected dose of tritium label down to 7.5% eight days after injection of this isotope. From this study both of these labels appear to be satisfactory for determining the erythrocyte lifespan of the horse. The mean time of the curve of mean radioselenium activity of peripheral blood leukocytes for the three horses was 5.54 days and the lifespan of these cells was seven days. The mean lifespan of peripheral blood leukocytes of the three horses after in vivo labelling with tritiated diisopropylfluorophosphate was 17.3 hours. No specific labelling was found in bone marrow or peripheral blood cells at this level of tritium labelling by dipping emulsion autoradiography. The mean lifespan of equine platelets for three horses using radioselenomethionine was 9.2 days and the mean lifespan of equine platelets using tritiated diisopropylfluorophosphate was 6.6 days in a group of three horses.
给3匹标准赛马静脉注射0.2毫克（1毫居里）的（75）硒代蛋氨酸，另一组3匹马静脉注射10毫居里的氚标记二异丙基氟磷酸酯（0.5毫克）。在注射放射性硒后持续观察标记细胞250天，注射氚后持续观察160天。使用（75）硒代蛋氨酸时红细胞的寿命为155±10天，使用氚标记二异丙基氟磷酸酯时为148±7.8天。使用这些标记物时，寿命在10%水平上无显著差异。注射标记物后82±18.9天，放射性硒进入红细胞的摄取平均最大值达到11.5%。注射该同位素8天后，氚标记物的洗脱量从注射剂量的17.6%降至7.5%。从这项研究来看，这两种标记物似乎都适合用于测定马的红细胞寿命。3匹马外周血白细胞平均放射性硒活性曲线的平均时间为5.54天，这些细胞的寿命为7天。用氚标记二异丙基氟磷酸酯体内标记后，3匹马外周血白细胞的平均寿命为17.3小时。通过浸入式乳胶放射自显影在该氚标记水平下，在骨髓或外周血细胞中未发现特异性标记。在一组3匹马中，使用放射性硒代蛋氨酸时马血小板的平均寿命为9.2天，使用氚标记二异丙基氟磷酸酯时马血小板的平均寿命为6.6天。
