Monette F C, DeMello J B
Cell Tissue Kinet. 1979 Mar;12(2):161-75. doi: 10.1111/j.1365-2184.1979.tb00123.x.
The seeding efficiency of colony-forming cells from normal, regenerating and velocity-sedimented cycling and non-cycling narrow preparations was compared. Colony-forming cells in cycle were found to exhibit a 50% reduction in splenic seeding when compared to normal marrow or sedimented non-cycling cells. The results of this study indicate that the spleen colony assay underestimates the total number of colony-forming cells by a fraction which is directly related to the number of cells in cycle.
比较了来自正常、再生以及经过速度沉降的循环和非循环狭窄制剂的集落形成细胞的接种效率。结果发现，与正常骨髓或沉降的非循环细胞相比，处于细胞周期中的集落形成细胞在脾脏接种时减少了50%。本研究结果表明，脾集落试验低估了集落形成细胞的总数，其低估的比例与处于细胞周期中的细胞数量直接相关。
