头孢菌素I对大鼠肾溶酶体稳定性的影响。

The effect of cephaloridine on the stability of rat kidney lysosomes.

作者信息

Ngaha E O, Fry M, Plummer D T

出版信息

Chem Biol Interact. 1979 Feb;24(2):199-208. doi: 10.1016/0009-2797(79)90008-5.

Abstract

The administration of cephaloridine to rats caused a decrease in the excretion of acid phosphatase into the urine. The antibiotic itself had no effect on urinary acid phosphatase and inhibitors or proteolytic enzymes were not present in the urine from treated rats. Cephaloridine may therefore be stabilizing the lysosomal membrane in vivo and experiments with isolated lysosomes confirm this hypothesis. The lysosomal integrity was followed by measuring the acid phosphatase activity and the light scattering properties of the particles. A good correlation was obtained between these parameters in the case of thermal disruption and progesterone induced lysis of the lysosomes and low concentrations of cephaloridine (0.1-1.0 mmol/1) protected the lysosomes against this form of damage.

摘要

给大鼠注射头孢菌素会导致其尿液中酸性磷酸酶的排泄量减少。抗生素本身对尿液酸性磷酸酶没有影响，且处理过的大鼠尿液中不存在蛋白酶抑制剂。因此，头孢菌素可能在体内稳定溶酶体膜，对分离出的溶酶体进行的实验证实了这一假设。通过测量酸性磷酸酶活性和颗粒的光散射特性来跟踪溶酶体的完整性。在溶酶体因热破坏和孕酮诱导裂解的情况下，这些参数之间存在良好的相关性，低浓度的头孢菌素（0.1 - 1.0 mmol/1）可保护溶酶体免受这种形式的损伤。