氯喹对大鼠肾溶酶体在体内和体外稳定性的影响。

Effect of chloroquine on the stability of rat kidney lysosomes in vivo and in vitro.

Ngaha E O, Akanji M A

Comp Biochem Physiol C Comp Pharmacol. 1982;73(1):109-13. doi: 10.1016/0306-4492(82)90176-9.

Abstract

Chronic administration of chloroquine to rats results in increased urinary excretion of lysosomal acid phosphatase, muramidase and cathepsin D. 2. Various concentrations of chloroquine caused lysosomal membrane swelling as shown by decrease of light absorbance in lysosomal suspensions. 3. Incubating lysosomal suspensions in the presence of chloroquine resulted in a marked lysosomal acid phosphatase release. 4. Addition of acetylsalicylic acid, a lysosomal membrane stabilizer, into a lysosomal suspension containing chloroquine, reduced the degree of lysosomal membrane swelling and acid phosphatase release. 5. The results suggest a labilizing effect of chloroquine on rat kidney lysosomes.

摘要

对大鼠长期施用氯喹会导致溶酶体酸性磷酸酶、溶菌酶和组织蛋白酶D的尿排泄增加。2. 如溶酶体悬浮液光吸收降低所示，不同浓度的氯喹会导致溶酶体膜肿胀。3. 在氯喹存在下孵育溶酶体悬浮液会导致溶酶体酸性磷酸酶显著释放。4. 向含有氯喹的溶酶体悬浮液中添加溶酶体膜稳定剂乙酰水杨酸，可降低溶酶体膜肿胀程度和酸性磷酸酶释放。5. 结果表明氯喹对大鼠肾脏溶酶体有不稳定作用。