Gaugas J M, Wright C, Curzen P
Br J Exp Pathol. 1974 Oct;55(5):478-86.
Heteroantisera were raised against human placental homogenate (PH), or microsomal fraction (MF) which assumingly contained much trophoblast plasma membrane. Anti-PH serum included a readily demonstrable antibody against a fairly soluble mucopolysaccharide (PMA) embodied in the placenta. Anti-MF serum contained antibodies against 4 demonstrable antigens (designated IPA-a, IPA-b, IPA-c and IPA-H) in solubilized MF. Solubilized MF was fractionated in order to partially isolate and characterize the antigens which were apparently glycoproteins (molecular weight range of 800,000-30,000), Whereas solubilization achieved in a Triton X-100 solution followed by sonication was virtually complete (yield 95-100%), papain enzymatic digestion (yield 40-50%) released proportionately more of the lower molecular weight components (160,000-30,000) which incorporated IPA-c and IPA-H antigens. Antigen IPA-H bound to concanavalin A and so could be partially purified by affinity chromatography. Antigens IPA-a, IPA-b and IPA-H were shared by kidney MF, but IPA-c and PMA might be tissue specific.
制备了针对人胎盘匀浆（PH）或微粒体部分（MF）的异种抗血清，假定微粒体部分含有大量滋养层细胞膜。抗PH血清包含一种易于证实的针对胎盘中所含相当易溶的粘多糖（PMA）的抗体。抗MF血清含有针对溶解的MF中4种可证实抗原（分别命名为IPA-a、IPA-b、IPA-c和IPA-H）的抗体。对溶解的MF进行分级分离，以便部分分离和鉴定明显为糖蛋白（分子量范围为800,000 - 30,000）的抗原。虽然在Triton X-100溶液中进行溶解并随后超声处理几乎可完全溶解（产率95 - 100%），但木瓜蛋白酶酶解（产率40 - 50%）会按比例释放更多包含IPA-c和IPA-H抗原的低分子量成分（160,000 - 30,000）。抗原IPA-H与伴刀豆球蛋白A结合，因此可通过亲和层析进行部分纯化。抗原IPA-a、IPA-b和IPA-H在肾MF中也有，但是IPA-c和PMA可能具有组织特异性。
