Johnson P M, Faulk W P
Clin Exp Immunol. 1976 Jun;24(3):435-40.
It has been demonstrated immunohistologically using cryostat sections that the lectins PHA and Con A bind to human placentae. Both lectins stain trophoblastic basement membrane (TBM), perivascular tissue and stroma. No staining of trophoblasts was observed. It has been shown by blocking experiments that the lectin binding sites on placental TBM are glycoproteins, whereas experiments involving pretreatment of placental sections with anti-collagen antisera or highly purified bacterial collagenase have indicated that lectin binding to stromal and perivascular structures is collagen-associated. The possible relation of TBM lectin-binding sites to immune response gene products is briefly discussed.
利用低温恒冷切片进行免疫组织学研究已证实，凝集素PHA和伴刀豆球蛋白A可与人胎盘结合。两种凝集素均能使滋养层基底膜（TBM）、血管周围组织和间质染色。未观察到滋养层细胞有染色现象。阻断实验表明，胎盘TBM上的凝集素结合位点是糖蛋白，而用抗胶原抗血清或高度纯化的细菌胶原酶对胎盘切片进行预处理的实验表明，凝集素与间质和血管周围结构的结合与胶原相关。本文简要讨论了TBM凝集素结合位点与免疫反应基因产物之间可能的关系。
