Tarrago-Litvak L, Desgranges C, Araya A, Litvak S
Eur J Biochem. 1979 Jan 15;93(2):271-8. doi: 10.1111/j.1432-1033.1979.tb12820.x.
Two different DNA polymerases have been purified and characterized from human platelets. In the mitochondrial fraction a unique activity of the polymerase gamma type has been found. The same enzyme is found in the extramitochondrial supernatant. A second DNA polymerase, called 'cytoplasmic' DNA polymerase has been found in the 10000 x g supernatant of human platelets. The following properties of the latter DNA polymerase from human platelets are identical to those of DNA polymerase alpha from normal cells: DEAE-cellulose and phosphocellulose chromatography, size, thermal stability, phosphonoacetic acid and ethidium bromide inhibition. However, some of its properties, like high resistance to N-ethylmaleimide and the lack of DNA polymerization using synthetic RNA primers, are those of DNA polymerase beta.
已从人血小板中纯化并鉴定出两种不同的DNA聚合酶。在线粒体部分发现了一种独特的γ型聚合酶活性。在胞质外线粒体上清液中也发现了相同的酶。在人血小板的10000×g上清液中发现了第二种DNA聚合酶，称为“细胞质”DNA聚合酶。来自人血小板的后一种DNA聚合酶的以下特性与正常细胞的DNA聚合酶α相同：二乙氨基乙基纤维素和磷酸纤维素色谱法、大小、热稳定性、膦酰乙酸和溴化乙锭抑制作用。然而，它的一些特性，如对N-乙基马来酰亚胺的高抗性以及使用合成RNA引物时缺乏DNA聚合作用，却是DNA聚合酶β的特性。
