Mous J, Peeters B, van Bellegem H, Rombauts W
Eur J Biochem. 1979 Mar;94(2):393-400. doi: 10.1111/j.1432-1033.1979.tb12906.x.
Polysomal RNA was extracted from human term placenta and total poly(A)-containing RNA purified by affinity chromatography on oligo(dT)-cellulose. Poly(A)-containing RNA constituted approximately 1.2% of the total polysomal RNA and 8% of this purified preparation was able to anneal with [3H]poly(U). When injected into Xenopus oocytes, this poly(A)-rich RNA directed the synthesis of a polypeptide which is immunoprecipitable with a specific antiserum to human placental lactogen. The identity of authentic human placental lactogen and the immunoreactive polypeptide synthesized in the oocytes is suggested by their identical behaviour in dodecylsulfate gel electrophoresis and by the formation of identical cyanogen bromide peptides. No precursor of human placental lactogen can be detected in the oocytes. The messenger RNA for human placental lactogen is very stable in oocytes; it is translated efficiently for a period of at least 7 days.
从足月人胎盘中提取多核糖体RNA，并通过寡聚（dT）-纤维素亲和层析纯化总含poly（A）RNA。含poly（A）RNA约占多核糖体RNA总量的1.2%，该纯化制剂的8%能够与[3H]聚尿苷酸退火。当将这种富含poly（A）的RNA注射到非洲爪蟾卵母细胞中时，它指导合成一种多肽，该多肽可用针对人胎盘催乳素的特异性抗血清进行免疫沉淀。真实的人胎盘催乳素与在卵母细胞中合成的免疫反应性多肽的一致性，由它们在十二烷基硫酸钠凝胶电泳中的相同行为以及相同溴化氰肽的形成所表明。在卵母细胞中未检测到人胎盘催乳素的前体。人胎盘催乳素的信使RNA在卵母细胞中非常稳定；它至少能有效翻译7天。
