Kopeyan C, Martinez G, Rochat H
Eur J Biochem. 1979 Mar;94(2):609-15. doi: 10.1111/j.1432-1033.1979.tb12931.x.
The amino acid sequence of neurotoxin III, purified from the venom of the North African scorpion Androctonus australis Hector, has been determined by Edman degradation using a liquid-phase sequencer. Carboxypeptidase A hydrolyses confirmed not only the sequence of the five last residues but also the presence of a free alpha-carboxylic group at the C-terminus. Edman degradation was conducted on one hand with the Quadrol [N,N,N',N'-tetrakis(2-hydroxypropyl)ethylene diamine] program and S-alkylated protein before or after coupling with sulfophenylisothiocynate (the first 34 residues were thus identified), on the other hand on tryptic and chymotryptic peptides with a dimethylbenzylamine program (residues 1--23 and 31--34 were confirmed, the positions of residues 35-64 were established). Neurotoxin III was found to belong to the same group of scorpion toxins active on mammals as neurotoxin I purified from the same venom (50 homologous positions exist in the two proteins).
从北非蝎子澳链尾蝎毒液中纯化得到的神经毒素III的氨基酸序列，已通过使用液相测序仪的埃德曼降解法确定。羧肽酶A水解不仅证实了最后五个残基的序列，还证实了C末端存在游离的α-羧基。一方面，在与磺苯基异硫氰酸酯偶联之前或之后，使用Quadrol [N,N,N',N'-四(2-羟丙基)乙二胺]程序和S-烷基化蛋白进行埃德曼降解（从而鉴定出前34个残基）；另一方面，使用二甲基苄胺程序对胰蛋白酶和胰凝乳蛋白酶肽段进行埃德曼降解（确认了残基1-23和31-34的位置，确定了残基35-64的位置）。发现神经毒素III与从同一种毒液中纯化得到的神经毒素I属于对哺乳动物有活性的同一类蝎子毒素（两种蛋白质中有50个同源位置）。
