用于微量肾素测定的兔肾素底物的制备。
The preparation of rabbit renin-substrate for the assay of minute amounts of renin.
作者信息
Cook W F, Lee M R
出版信息
Biochem J. 1965 Aug;96(2):413-8. doi: 10.1042/bj0960413.
Abstract
- By DEAE-cellulose chromatography and treatment with Cd(2+) a partially purified renin-substrate has been prepared from rabbit serum. 2. It has the following properties: (a) Angiotensinase activity is undetectable: 0.1mug. of angiotensin was recovered intact after incubation with the substrate at 37 degrees for 200hr. (b) It does not contain renin and does not generate vaso-active substances when incubated alone. (c) It is ten times as active as serum in terms of angiotensin produced/mg. of protein. (d) It has not deteriorated after storage for 3 months at 4 degrees at pH7.5.
摘要
- 通过二乙氨基乙基纤维素色谱法和用镉离子处理，已从兔血清中制备出部分纯化的肾素底物。2. 它具有以下特性：(a) 未检测到血管紧张素酶活性：在37℃与底物孵育200小时后，回收的完整血管紧张素为0.1微克。(b) 它不含肾素，单独孵育时不产生血管活性物质。(c) 就每毫克蛋白质产生的血管紧张素而言，其活性是血清的十倍。(d) 在pH7.5条件下于4℃储存3个月后，它并未变质。
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