兔血浆中肾素底物的特性及其测定方法的说明
Properties of renin substrate in rabbit plasma with a note on its assay.
作者信息
Ryan J W, McKenzie J K
出版信息
Biochem J. 1968 Jul;108(4):687-92. doi: 10.1042/bj1080687.
Abstract
- Rabbit plasma enzymes that degrade angiotensin I are inhibited completely by the combination of 2,3-dimercaptopropan-1-ol (10mm), EDTA (10mm) and chlorhexidine gluconate (0.005%, w/v). These compounds do not modify the reaction of renin with renin substrate and are termed the selective inhibitors. 2. The renin substrate concentration of plasma can be measured as angiotensin I content by incubating plasma plus the selective inhibitors with renin for a time sufficient to allow complete utilization of renin substrate. 3. This reaction obeys first-order kinetics to substrate concentrations of at least 1000ng. of angiotensin I content/ml. In general, the renin substrate concentrations of normal rabbit plasmas are less than 1000ng. of angiotensin I content/ml. Thus the time required for the complete release of angiotensin I from normal plasma is inversely related to renin activity and is independent of renin substrate concentration. 4. A method for the assay of renin substrate, taking these reaction kinetics into account, is presented.
摘要
- 2,3 - 二巯基丙醇（10mmol）、乙二胺四乙酸（10mmol）和葡萄糖酸氯己定（0.005%，w/v）的组合可完全抑制兔血浆中降解血管紧张素I的酶。这些化合物不会改变肾素与肾素底物的反应，被称为选择性抑制剂。2. 通过将血浆与选择性抑制剂一起孵育，再加入肾素一段足以使肾素底物完全被利用的时间，血浆中的肾素底物浓度可以通过血管紧张素I含量来测量。3. 该反应对至少1000ng血管紧张素I含量/毫升的底物浓度遵循一级动力学。一般来说，正常兔血浆中的肾素底物浓度低于1000ng血管紧张素I含量/毫升。因此，从正常血浆中完全释放血管紧张素I所需的时间与肾素活性呈负相关，且与肾素底物浓度无关。4. 提出了一种考虑这些反应动力学的肾素底物测定方法。
相似文献
1
Properties of renin substrate in rabbit plasma with a note on its assay.兔血浆中肾素底物的特性及其测定方法的说明
Biochem J. 1968 Jul;108(4):687-92. doi: 10.1042/bj1080687.
2
A rapid simple method for the assay of renin in rabbit plasma.一种快速简便的兔血浆肾素测定方法。
Biochem J. 1968 Jul;108(4):679-85. doi: 10.1042/bj1080679.
3
Measurement of renin concenration in human plasma using 125i-labelled sheep renin substrate.使用125I标记的绵羊肾素底物测定人血浆中的肾素浓度。
Clin Chim Acta. 1975 Dec 15;65(3):361-70. doi: 10.1016/0009-8981(75)90262-4.
4
Angiotensin and renin in rat and dog brain.大鼠和犬脑中的血管紧张素与肾素
J Exp Med. 1971 Feb 1;133(2):353-61. doi: 10.1084/jem.133.2.353.
5
A plasma inhibitor of the renin-antirenin reaction and the in vitro generation of angiotensin I.
Metabolism. 1975 Feb;24(2):127-38. doi: 10.1016/0026-0495(75)90013-x.
6
Purification, properties and kinetics of sheep and human renin substrates.绵羊和人肾素底物的纯化、性质及动力学
Aust J Exp Biol Med Sci. 1975 Feb;53(1):77-88. doi: 10.1038/icb.1975.8.
7
The effect of mercuric chloride, of bilateral ureteral ligation and of bilateral nephrectomy on plasma renin substrate concentration in rats.氯化汞、双侧输尿管结扎及双侧肾切除对大鼠血浆肾素底物浓度的影响。
Jpn Circ J. 1968 Nov;32(11):1591-5. doi: 10.1253/jcj.32.1591.
8
Lack of inhibition of hog renin by heparin.
Nephron. 1978;22(1-3):113-6. doi: 10.1159/000181430.
9
Kinetics of renin-angiotensinogen reaction in plasma of normal and nephrectomized rats.正常大鼠和肾切除大鼠血浆中肾素-血管紧张素原反应的动力学
Am J Physiol. 1968 Jul;215(1):78-83. doi: 10.1152/ajplegacy.1968.215.1.78.
10
Problems connected with plasma renin activity measurements by angiotensin I radioimmunoassay.
Horm Metab Res. 1975 Jul;7(4):342-7. doi: 10.1055/s-0028-1093727.
引用本文的文献
1
Capillaries of the adrenal cortex possess aminopeptidase A and angiotensin-converting-enzyme activities.肾上腺皮质的毛细血管具有氨肽酶A和血管紧张素转换酶活性。
Biochem J. 1980 Feb 15;186(2):605-8. doi: 10.1042/bj1860605.
2
Content of the renin-like enzyme in pregnant and non-pregnant rabbit uterus.怀孕和未怀孕兔子子宫中肾素样酶的含量
Experientia. 1971 Dec 15;27(12):1391-3. doi: 10.1007/BF02154247.
3
Effect of renal nerve stimulation, renal blood flow and adrenergic blockade on plasma renin activity in the cat.肾神经刺激、肾血流量及肾上腺素能阻断对猫血浆肾素活性的影响。
J Physiol. 1972 Oct;226(1):15-36. doi: 10.1113/jphysiol.1972.sp009971.
4
Distribution of a renin-like enzyme in the bovine adrenal gland.一种肾素样酶在牛肾上腺中的分布。
Experientia. 1973 Apr 15;29(4):407-8. doi: 10.1007/BF01926748.
5
Vascular resistance in the perfused isolated rat tail.灌注离体大鼠尾部的血管阻力。
Br J Pharmacol. 1970 Jan;38(1):20-36. doi: 10.1111/j.1476-5381.1970.tb10333.x.
6
A rapid simple method for the assay of renin in rabbit plasma.一种快速简便的兔血浆肾素测定方法。
Biochem J. 1968 Jul;108(4):679-85. doi: 10.1042/bj1080679.
7
Synthesis of renin substrate by rat liver.大鼠肝脏中肾素底物的合成
Biochem J. 1979 Oct 15;184(1):7-12. doi: 10.1042/bj1840007.
本文引用的文献
1
The purification and partial characterization of several forms of hog renin substrate.几种形式的猪肾素底物的纯化及部分特性分析
J Exp Med. 1963 Jul;118(1):73-98. doi: 10.1084/jem.118.1.73.
2
The amino acid sequence in a hypertensin.高血压素中的氨基酸序列。
Biochem J. 1957 Feb;65(2):246-54. doi: 10.1042/bj0650246.
3
The amino acid sequence of hypertensin. II.高血压素II的氨基酸序列。二。
J Exp Med. 1956 Aug 1;104(2):193-7. doi: 10.1084/jem.104.2.193.
4
The amino acid composition of hypertensin II and its biochemical relationship to hypertensin I.血管紧张素II的氨基酸组成及其与血管紧张素I的生化关系。
J Exp Med. 1956 Aug 1;104(2):183-91. doi: 10.1084/jem.104.2.183.
5
The isolation of a hypertension.一种高血压的隔离。 （此译文可能不太符合准确语境含义，原句表述较奇怪，也许是 “The isolation of a hypertensive patient”之类表述的错误形式）
Biochem J. 1956 Mar;62(3):520-7. doi: 10.1042/bj0620520.
6
The preparation and function of the hypertensin-converting enzyme.血管紧张素转换酶的制备与功能。
J Exp Med. 1956 Mar 1;103(3):295-9. doi: 10.1084/jem.103.3.295.
7
The existence of two forms of hypertensin.高血压素两种形式的存在。
J Exp Med. 1954 Mar;99(3):275-82. doi: 10.1084/jem.99.3.275.
8
The gel-filtration behaviour of proteins related to their molecular weights over a wide range.蛋白质的凝胶过滤行为与其在很宽范围内的分子量相关。
Biochem J. 1965 Sep;96(3):595-606. doi: 10.1042/bj0960595.
9
Purification of pig renin.
Biochem J. 1966 Jun;99(3):708-16. doi: 10.1042/bj0990708.
10
Studies on the preparation and properties of renin.肾素的制备及其性质研究。
Circ Res. 1967 Jul;21(1):Suppl 2:91+.
Zotero 插件