Vogler W R, Winton E F, Guzman G
Exp Hematol. 1979 Jan;7(1):27-35.
Urinary granulocyte colony stimulating activity (CSA) was studied in normal individulas donating granulocytes. Donors were given corticosteroids 2 h prior to a 4-h leukapheresis using an Aminco celltrifuge in which hydroxyethyl starch was introduced into the donor line. Urine was collected 12-24 h prior to the procedure and 12-24 h beginning at the time of administration of corticosteroids. Colony stimulating activity was measured using mouse marrow cells grown in soft agar. After leukapheresis a significant increase in protein excretion was noted (139.94 +/- 28.1 to 288.69 +/- 63.8 mg per gram of creatinine) and the bulk of the protein was albumin. CSA isolated from G-75 Sephadex columns was increased in five donors, decreased in five donors and undetectable in nine donors. No CSA inhibitors were detectable. There was a significant correlation between the quantity of protein recovered from G-75 Sephadex column and CSA.
对捐献粒细胞的正常个体的尿粒细胞集落刺激活性（CSA）进行了研究。在使用Aminco细胞离心机进行4小时白细胞单采前2小时，给供体注射皮质类固醇，在供体管路中加入羟乙基淀粉。在操作前12 - 24小时以及从注射皮质类固醇时开始的12 - 24小时收集尿液。使用在软琼脂中生长的小鼠骨髓细胞测量集落刺激活性。白细胞单采后，蛋白质排泄显著增加（从每克肌酐139.94±28.1增加到288.69±63.8毫克），且大部分蛋白质为白蛋白。从G - 75 Sephadex柱分离的CSA在5名供体中增加，在5名供体中减少，在9名供体中未检测到。未检测到CSA抑制剂。从G - 75 Sephadex柱回收的蛋白质量与CSA之间存在显著相关性。
