Hauschild A H
J Bacteriol. 1965 Dec;90(6):1569-74. doi: 10.1128/jb.90.6.1569-1574.1965.
Hauschild, Andreas H. W. (University of Toronto, Toronto, Ontario, Canada). Incorporation of C(14) from amino acids and peptides into protein by Clostridium perfringens type D. J. Bacteriol. 90:1569-1574. 1965.-Uptake of C(14) from C(14)-labeled amino acids and peptides by Clostridium perfringens was measured in culture media containing acid or papain hydrolysates of C(14)-labeled Chlorella protein. Between 2 and 4 hr of growth, the rate of C(14) uptake from peptides was higher than from free amino acids. Peptides extracted from cells with hot ethyl alcohol contained six to nine times more C(14) after 4 hr of growth with C(14)-labeled peptides than with C(14)-labeled amino acids. Incorporation of C(14)-labeled glycine, serine, threonine, alanine, and proline into both cellular and exocellular protein was two to five times higher when these were supplied as components of dialyzable peptides rather than as free amino acids.
豪施尔德，安德烈亚斯·H.W.（加拿大多伦多大学，安大略省多伦多）。D型产气荚膜梭菌将氨基酸和肽中的碳-14掺入蛋白质。《细菌学杂志》90:1569 - 1574。1965年。——在含有碳-14标记小球藻蛋白的酸水解物或木瓜蛋白酶水解物的培养基中，测定了D型产气荚膜梭菌对碳-14标记的氨基酸和肽中碳-14的摄取情况。在生长2至4小时期间，从肽中摄取碳-14的速率高于从游离氨基酸中摄取的速率。在用碳-14标记的肽生长4小时后，用热乙醇从细胞中提取的肽所含的碳-14比用碳-14标记的氨基酸生长时多6至9倍。当碳-14标记的甘氨酸、丝氨酸、苏氨酸、丙氨酸和脯氨酸作为可透析肽的成分而非游离氨基酸提供时，其掺入细胞内和细胞外蛋白质的量高出2至5倍。
