Sebald M, Costilow R N
Appl Microbiol. 1975 Jan;29(1):1-6. doi: 10.1128/am.29.1.1-6.1975.
The minimal growth requirements for two strains of Clostridium perfringens were defined, and both synthetic and semisynthetic plating media were developed. Plate counts of the wild-type strains on both of these minimal media were equivalent to those on complex media. A number of auxotrophic mutants of each strain were isolated, and their phenotypes were defined.
确定了两株产气荚膜梭菌的最低生长需求,并开发了合成和半合成平板培养基。这两种基本培养基上野生型菌株的平板计数与复杂培养基上的相当。分离出了每株菌的一些营养缺陷型突变体,并确定了它们的表型。