Kende M, Robbins M L
Appl Microbiol. 1965 Nov;13(6):1026-9. doi: 10.1128/am.13.6.1026-1029.1965.
Modification of the micro tissue culture technique, including incubation under increased CO(2), resulted in prolongation of the viability of the cells. As a consequence, satisfactory titrations of poliovirus and of poliovirus antiserum have been achieved by the micro method. The technique offers a number of advantages over conventional methods.