Gabrielson M O, Hsiung G D
Appl Microbiol. 1965 Nov;13(6):967-72. doi: 10.1128/am.13.6.967-972.1965.
(i) for isolation of enterovirus when the number of virus particles was too small to produce detectable cytopathic effect (CPE) in fluid cultures, (ii) for isolation of echovirus 22 which did not produce detectable CPE in fluid cultures, (iii) as an aid to rapid differentiation of enteroviruses, and (iv) for differentiation of viruses in mixed infections. Nonpolio enterovirus isolation experience in the New Haven area over a 4-year period is presented. It was concluded that the agar overlay technique is both useful and relatively simple for routine examination of clinical specimens in a diagnostic laboratory.
将琼脂覆盖技术与液体培养技术用于肠道病毒的分离和鉴定进行比较，结果表明前者具有以下用途：(i) 当病毒颗粒数量过少以至于在液体培养中无法产生可检测到的细胞病变效应 (CPE) 时用于分离肠道病毒；(ii) 用于分离在液体培养中不产生可检测到的CPE的埃可病毒22；(iii) 作为辅助快速区分肠道病毒的方法；(iv) 用于区分混合感染中的病毒。本文介绍了纽黑文地区4年期间非脊髓灰质炎肠道病毒的分离经验。得出的结论是，琼脂覆盖技术对于诊断实验室临床标本的常规检查既有用又相对简单。
