Wecker I, ter Meulen V
Med Microbiol Immunol. 1977 Dec 27;163(4):233-40. doi: 10.1007/BF02125507.
It is shown that, in addition to the already known replication of Coxsackie A viruses, 16 out of 18 different types of ECHO viruses tested as well as all three types of polioviruses can also be grown in RD cells with the development of a pronounced CPE. One of the exceptions, ECHO virus type 16, can be propagated in either HEL or HFDK cells and the other, ECHO virus type 22, in Vero cells. These four permanent or semi-permanent cell cultures thus prove very useful for the isolation of most of the diagnostically important enteroviruses as well as for their serological identification by neutralization in a micro-titer system described in this paper.
结果表明，除了已知的柯萨奇A病毒能复制外，所检测的18种不同类型的埃可病毒中有16种以及所有三种脊髓灰质炎病毒也能在RD细胞中生长，并产生明显的细胞病变效应（CPE）。其中一个例外是16型埃可病毒，它可以在人胚肺成纤维细胞（HEL）或人包皮成纤维细胞（HFDK）中增殖；另一个例外是22型埃可病毒，它可以在非洲绿猴肾细胞（Vero）中增殖。因此，这四种永久或半永久细胞培养物对于分离大多数具有诊断意义的肠道病毒以及通过本文所述的微量滴定系统中的中和试验进行血清学鉴定非常有用。
