Platt D, Gross-Fengels F
Gerontology. 1979;25(2):87-93. doi: 10.1159/000212325.
Measurements of DNA and protein content and determinations of lysosomal enzyme activities (beta-glucuronidase, beta-acetylglucosaminidase, cathepsin D) were performed in the rat liver following partial hepatectomy and spironolactone administration. The rats (SIV) were divided into three age-groups: 6 weeks old, 10 months old and 18 months old. 60 h after the administration of spironolactone (2 x 10 mg Aldactone/100 g body weight/day orally via gastric tube for 3 days) partial hepatectomy was performed. The removed two thirds of the liver and the regenerating liver (24 h after partial hepatectomy) were prepared for the biochemical examinations at 4 degrees C. The measurements of the enzyme activities were performed in liver homogenates. The results indicate that the administration of spironolactone cause (1) a weight increase in the normal as well as in the regenerating liver, especially in the older animals; (2) an increase of the protein content of the normal liver in the oldest age-group; (3) a decrease in the DNA content of the normal and regenerating liver of old rats, and (4) an age-dependent increase of decrease of the three lyososomal enzymes beta-glucuronidase, beta-acetylglucosamidase and cathepsin D.
在大鼠部分肝切除并给予螺内酯后，对其肝脏进行了DNA和蛋白质含量测定以及溶酶体酶活性（β-葡萄糖醛酸酶、β-乙酰氨基葡萄糖苷酶、组织蛋白酶D）的测定。大鼠（SIV）被分为三个年龄组：6周龄、10月龄和18月龄。在通过胃管口服给予螺内酯（2×10mg安体舒通/100g体重/天，连续3天）60小时后进行部分肝切除。切除的肝脏的三分之二以及再生肝脏（部分肝切除后24小时）在4℃下制备用于生化检查。酶活性测定在肝脏匀浆中进行。结果表明，给予螺内酯会导致：（1）正常肝脏以及再生肝脏重量增加，尤其是在老年动物中；（2）最老龄组正常肝脏的蛋白质含量增加；（3）老年大鼠正常肝脏和再生肝脏的DNA含量降低；（4）三种溶酶体酶β-葡萄糖醛酸酶、β-乙酰氨基葡萄糖苷酶和组织蛋白酶D的活性随年龄增长而出现增减变化。
