[再生肝组织的溶酶体神经氨酸酶、β-半乳糖苷酶和β-N-乙酰氨基葡萄糖苷酶]

[Lysosomal neuraminidase, beta-galactosidase and beta-N-acetylglucosaminidase of regenerating liver tissue].

作者信息

Valiakina T I, Gabrielian N D, Komaleva R L, Khorlin A Ia

出版信息

Biokhimiia. 1977 Mar;42(3):513-8.

Abstract

Neuraminidase (EC 3.2.1.18) beta-galactosidase (EC 3.2.1.23) and beta-N-acetylglucosaminidase (EC 3.2.1.30) were studied in normal and regenerating rat liver. All these glycosidases were shown to be predominantly localized in lysosome rich fraction. The activity of lysosomal neuraminidase in regenerating rat liver increased 24 hours after partial hepatectomy, whereas those of beta-galactosidase and beta-N-acetylglucosaminidase decreased. The presence of inhibitor of neurominidase in regenerating liver homogenate was shown. Actinomycin D and cycloheximide were shown to have no significant effect on lysosomal neuraminidase in regenerating rat liver.

摘要

在正常和再生的大鼠肝脏中研究了神经氨酸酶（EC 3.2.1.18）、β-半乳糖苷酶（EC 3.2.1.23）和β-N-乙酰氨基葡萄糖苷酶（EC 3.2.1.30）。所有这些糖苷酶主要定位于富含溶酶体的部分。部分肝切除术后24小时，再生大鼠肝脏中溶酶体神经氨酸酶的活性增加，而β-半乳糖苷酶和β-N-乙酰氨基葡萄糖苷酶的活性降低。结果表明再生肝匀浆中存在神经氨酸酶抑制剂。放线菌素D和环己酰亚胺对再生大鼠肝脏中的溶酶体神经氨酸酶无显著影响。