奥氏甲烷杆菌提取物将甲基钴胺素转化为CH-4所需的成分。

Components required for the formation of CH-4 from methylcobalamin by extracts of Methanobacillus omelianskii.

作者信息

Wood J M, Wolfe R S

出版信息

J Bacteriol. 1966 Sep;92(3):696-700. doi: 10.1128/jb.92.3.696-700.1966.

DOI:10.1128/jb.92.3.696-700.1966

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC276310/

Abstract

Wood, J. M. (University of Illinois, Urbana), and R. S. Wolfe. Components required for the formation of CH(4) from methylcobalamin by extracts of Methanobacillus omelianskii. J. Bacteriol. 92:696-700. 1966.-Optimal conditions for the formation of CH(4) from methylcobalamin (methyl-cobalt-5,6-dimethylbenzimidazolylcobamide) by partially purified extracts of Methanobacillus omelianskii (Methanobacterium omelianskii) were achieved by the addition of two protein fractions, adenosine triphosphate, Mg(++), and a reduced flavin adenine dinucleotide-generating system. Adenosine diphosphate and adenosine monophosphate play an important role in the regulation of C(1) transfer in this reaction. The possible position of adenosine triphosphate in B(12)-dependent methyl transfer is discussed.

摘要

伍德，J.M.（伊利诺伊大学厄巴纳分校）和R.S.沃尔夫。奥氏甲烷杆菌提取物由甲基钴胺素形成甲烷所需的成分。《细菌学杂志》92:696 - 700。1966年。- 通过添加两种蛋白质组分、三磷酸腺苷、Mg(++)以及一个还原型黄素腺嘌呤二核苷酸生成系统，实现了奥氏甲烷杆菌（奥氏甲烷菌）部分纯化提取物由甲基钴胺素（甲基 - 钴 - 5,6 - 二甲基苯并咪唑基钴胺酰胺）形成甲烷的最佳条件。二磷酸腺苷和一磷酸腺苷在该反应中碳（1）转移的调节中起重要作用。讨论了三磷酸腺苷在依赖维生素B12的甲基转移中的可能位置。

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