5'-单磷酸腺苷对巴氏甲烷八叠球菌细胞提取物中5'-三磷酸腺苷激活甲基辅酶M甲基还原酶的影响。
Effect of adenosine 5'-monophosphate on adenosine 5'-triphosphate activation of methyl coenzyme M methylreductase in cell extracts of Methanosarcina barkeri.
作者信息
Mountfort D O
出版信息
J Bacteriol. 1980 Aug;143(2):1039-41. doi: 10.1128/jb.143.2.1039-1041.1980.
Abstract
In cell extracts of Methanosarcina barkeri, adenosine 5'-triphosphate (ATP)-activated methyl coenzyme M methylreductase was inhibited by adenosine 5'-monophosphate (AMP) but not by cyclic AMP. AMP (2 and 4 mM) shifted the saturation curve for ATP activation from hyperbolic (Hill coefficient [n] = 1.0) to sigmoidal (n = 1.5), decreased Vmax, and increased the apparent KmATP.
摘要
在巴氏甲烷八叠球菌的细胞提取物中，腺苷5'-三磷酸（ATP）激活的甲基辅酶M甲基还原酶受到腺苷5'-单磷酸（AMP）的抑制，但不受环磷酸腺苷（cAMP）的抑制。AMP（2 mM和4 mM）使ATP激活的饱和曲线从双曲线（希尔系数[n]=1.0）转变为S形曲线（n = 1.5），降低了最大反应速度（Vmax），并增加了ATP的表观米氏常数（KmATP）。
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