来自大鼠肝脏的快速标记核糖核酸。关于在无细胞系统中与核糖体的附着以及对氨基酸掺入多肽的刺激作用的研究。
Rapidly labelled ribonucleic acid from rat liver. Studies in the attachment to ribosomes and stimulation of the incorporation of amino acids into polypeptides in cell-free systems.
作者信息
Wilkinson B R, Kirby K S
出版信息
Biochem J. 1966 Jun;99(3):786-92. doi: 10.1042/bj0990786.
Abstract
- Rapidly labelled RNA from rat liver, either as a complex with DNA (m-RNA-DNA) or with ribosomal RNA (m-RNA-RNA) binds to ribosomes in the polysome region. No binding could be demonstrated with ribosomal RNA or native DNA from Bacillus subtilis. 2. With ribosomes from rat liver, Escherichia coli or hepatoma the m-RNA-DNA stimulated incorporation of amino acids with rat-liver ribosomes only, whereas the m-RNA-RNA complex was effective with ribosomes from E. coli or the hepatoma. 3. Polyuridylic acid was effective as messenger RNA with all three ribosomes but much greater stimulation was obtained with ribosomes from E. coli and the hepatoma. 4. The degree of incorporation of phenylalanine with polyuridylic acid and ribosomes from a hepatoma was decreased by about 50% when ribosomal RNA was present.
摘要
- 来自大鼠肝脏的快速标记RNA，无论是与DNA形成复合物（m-RNA-DNA）还是与核糖体RNA形成复合物（m-RNA-RNA），都会与多核糖体区域的核糖体结合。未证明其与枯草芽孢杆菌的核糖体RNA或天然DNA有结合。2. 对于来自大鼠肝脏、大肠杆菌或肝癌的核糖体，m-RNA-DNA仅刺激大鼠肝脏核糖体掺入氨基酸，而m-RNA-RNA复合物对来自大肠杆菌或肝癌的核糖体有效。3. 多聚尿苷酸作为信使RNA对所有三种核糖体均有效，但对来自大肠杆菌和肝癌的核糖体有更大的刺激作用。4. 当存在核糖体RNA时，肝癌核糖体与多聚尿苷酸掺入苯丙氨酸的程度降低约50%。
相似文献
1
Rapidly labelled ribonucleic acid from rat liver. Studies in the attachment to ribosomes and stimulation of the incorporation of amino acids into polypeptides in cell-free systems.来自大鼠肝脏的快速标记核糖核酸。关于在无细胞系统中与核糖体的附着以及对氨基酸掺入多肽的刺激作用的研究。
Biochem J. 1966 Jun;99(3):786-92. doi: 10.1042/bj0990786.
2
Studies in rapidly labelled ribonucleic acid in cell fractions and in tumour tissues.细胞组分及肿瘤组织中快速标记核糖核酸的研究
Biochem J. 1966 Jun;99(3):780-5. doi: 10.1042/bj0990780.
3
4
Stimulation of 14C-leucine incorporation into protein in vitro by rapidly sedimenting nucleolar RNA from AH-130 hepatoma cells.
J Biochem. 1969 Dec;66(6):867-9. doi: 10.1093/oxfordjournals.jbchem.a129218.
5
Studies on the role of 23 s nucleolar RNA as an intermediate in the synthesis of 18 s ribosomal RNA.关于23 s核仁RNA作为18 s核糖体RNA合成中间体作用的研究。
J Mol Biol. 1971 Mar 28;56(3):565-77. doi: 10.1016/0022-2836(71)90402-5.
6
RNA fractions in normal rat liver and Morris 5123D hepatoma.
Biochim Biophys Acta. 1968 Oct 29;166(3):656-62. doi: 10.1016/0005-2787(68)90372-9.
7
Arginine incorporation into proteins by supernatant fractions of rat liver and Novikoff hepatoma.精氨酸通过大鼠肝脏和诺维科夫肝癌的上清液部分掺入蛋白质中。
Cancer Res. 1970 May;30(5):1506-11.
8
[Problems posed by the biosynthesis of proteins in acellular systems of animal origin. Compariosn of the E. coli and rat liver systems].[动物源无细胞系统中蛋白质生物合成所带来的问题。大肠杆菌和大鼠肝脏系统的比较]
Bull Soc Chim Biol (Paris). 1968;50(4):773-93.
9
Effect of chloramphenicol on the synthesis and stability of ribonucleic acid in Bacillus subtilis.氯霉素对枯草芽孢杆菌核糖核酸合成及稳定性的影响。
J Bacteriol. 1968 Apr;95(4):1212-20. doi: 10.1128/jb.95.4.1212-1220.1968.
10
Ribosome formation in rat liver: evidence for post-transcriptional control.大鼠肝脏中的核糖体形成：转录后调控的证据。
Biochim Biophys Acta. 1969 Nov 19;195(1):109-22. doi: 10.1016/0005-2787(69)90607-8.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Characteristics and stabilization of DNAase-sensitive protein synthesis in E. coli extracts.大肠杆菌提取物中脱氧核糖核酸酶敏感蛋白合成的特性与稳定性
Proc Natl Acad Sci U S A. 1961 Oct 15;47(10):1580-8. doi: 10.1073/pnas.47.10.1580.
3
ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID.核糖体核糖核酸的分离与鉴定
Biochem J. 1965 Jul;96(1):266-9. doi: 10.1042/bj0960266.
4
STUDIES ON NUCLEIC ACIDS DURING CARCINOGENESIS.致癌过程中的核酸研究。
Br J Cancer. 1964 Dec;18(4):792-800. doi: 10.1038/bjc.1964.92.
5
THE STABILITY OF LIVER MESSENGER RNA.肝脏信使核糖核酸的稳定性
Proc Natl Acad Sci U S A. 1964 May;51(5):810-8. doi: 10.1073/pnas.51.5.810.
6
MESSENGER RIBONUCLEIC ACID IN RAT LIVER NUCLEI AND CYTOPLASM.大鼠肝脏细胞核与细胞质中的信使核糖核酸
J Mol Biol. 1964 Apr;8:479-88. doi: 10.1016/s0022-2836(64)80005-x.
7
BACTERIAL POLYRIBOSOMES AND THEIR PARTICIPATION IN PROTEIN SYNTHESIS IN VIVO.细菌多核糖体及其在体内蛋白质合成中的作用
J Mol Biol. 1963 Nov;7:561-8. doi: 10.1016/s0022-2836(63)80102-3.
8
A RIBONUCLEIC ACID FRACTION FROM RAT LIVER WITH TEMPLATE ACTIVITY.来自大鼠肝脏的具有模板活性的核糖核酸组分。
Proc Natl Acad Sci U S A. 1963 Oct;50(4):630-8. doi: 10.1073/pnas.50.4.630.
9
ISOLATION CHARACTERISTICS OF RAPIDLY LABELLED RNA FROM NORMAL RAT LIVER.正常大鼠肝脏快速标记RNA的分离特性
J Mol Biol. 1963 Sep;7:312-5. doi: 10.1016/s0022-2836(63)80010-8.
10
PROTEIN SYNTHESIS BY FREE AND BOUND RAT LIVER RIBOSOMES IN VIVO AND IN VITRO.大鼠肝脏游离核糖体和附着核糖体在体内及体外的蛋白质合成
J Mol Biol. 1963 Aug;7:122-9. doi: 10.1016/s0022-2836(63)80041-8.
Zotero 插件